You Searched For: N,N\'-Diisopropyl-O-(4-nitrobenzyl)isourea

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N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea 96%

Supplier: Ambeed

Description: N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea 96%

N,N'-Diisopropyl-O-(4-nitrobenzyl)isourea ≥95.0% (by HPLC)

Catalog Number: (TCA5506-001G)

Supplier: TCI America

Description: [for HPLC Labeling]
CAS Number: 2978-11-2
MDL Number: MFCD00042046
Molecular Formula: C14H21N3O3
Molecular Weight: 279.34
Purity/Analysis Method: >95.0% (HPLC)
Form: Crystal
Melting point (°C): 44
Storage Temperature: 0-10°C

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), No-Weigh™ Format, Pierce™

Catalog Number: (103523-940)

Supplier: Invitrogen

Description: Thermo Scientific Pierce EDC is a carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with a carboxyl group first and forms an amine-reactive O-acylisourea intermediate that quickly reacts with an amino group to form an amide bond with release of an isourea by-product. The intermediate is unstable in aqueous solutions and so two-step conjugation procedures rely on N-hydroxysuccinimide (NHS) for stabilization. Failure to react with an amine will result in hydrolysis of the intermediate, regeneration of the carboxyl, and release of an N-substituted urea. A side reaction is the formation of an N-acylurea, which is usually restricted to carboxyls located in hydrophobic regions of proteins.

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EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride) crosslinker

Supplier: G-Biosciences

Description: EDC is a heterobifunctional, water-soluble, zero-length carbodiimide crosslinker that is used to couple carboxyl groups to primary amines. EDC activates carboxyl groups first and forms amine reactive O-acylisourea imtermediate that spontaneously reacts with primary amines to form an amide bond and isourea by-product.

EDC-HCl (N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride), Pierce™

Supplier: Invitrogen

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Urea ≥99%, white prills, Ultrapure

Catalog Number: (76180-662)

Supplier: MP Biomedicals

Description: Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Urea is a mild agent usually used in the solubilization and denaturation of proteins. It is also useful for renaturing proteins from samples already denatured with 6 M guanidine hydrochloride such as inclusion bodies; and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose; used as a diuretic; enhances the action of sulfonamides; an antiseptic.
Urea in solution is in equilibrium with ammonium cyanate. The form that reacts with protein amino groups is isocyanic acid. Urea in the presence of heat and protein leads to carbamylation of the proteins. Carbamylation by isocyanic acid interferes with protein characterization because isocyanic acid reacts with the amino terminus of proteins, preventing N-terminal sequencing. Isocyanic acid also reacts with side chains of lysine and arginine residues resulting in a protein that is unsuitable for many enzymatic digests. In addition, carbamylation often leads to confusing results from peptides having unexpected retention times and masses. When performing enzymatic protein digests it is important to remove urea first. Even though some enzymes will tolerate small amounts of urea, the elevated temperature used for most reactions will lead to carbamylation during the course of the digest. The urea can be removed prior to digestion by fast reversed phase chromatography, spin columns, or dialysis.
Dissolve urea in deionized water to the desired concentration.For every 10 ml of solution, add 1 g of Amberlite® IRA-910.Stir for one hour at room temperature

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Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Diisopropyl ether ≥99.0% (by GC, corrected for water content) stabilized, BAKER ANALYZED® ACS, J.T.Baker®

Catalog Number: (JT9243-22)

Supplier: AVANTOR PERFORMANCE MATERIALS US

Description: Stabilized with approximately 0.01% hydroquinone. Lot analysis on label.

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Diisopropyl succinate 98%

Supplier: Ambeed

Description: Diisopropyl succinate 98%

Diisopropyl fluorophosphate 99%

Catalog Number: (TS11523-0010)

Supplier: Thermo Scientific Chemicals

Description: Diisopropyl fluorophosphate 99%

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Diisopropyl phosphonate ≥98%

Supplier: BeanTown Chemical

Description: CAS: 1809-20-7; EC No: 217-317-7; MDL No: MFCD00117905; RTECS: SZ7660000 Liquid; Molecular Formula: C6H15O3P; MW: 166.16 Boiling Point: 72-75°/10 mmHg Density (g/mL): 0.990 Moisture Sensitive

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Diisopropyl phthalate 98%

Supplier: Ambeed

Description: Diisopropyl Phthalate, Purity: 98%, CAS Number: 605-45-8, Appearance: Solid or Semi-solid or liquid, Storage: Sealed in dry, Room Temperature, Size: 5g