You Searched For: Phenyl(phenylamino)acetic+acid

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Dihydrorhodamine 123

Supplier: Adipogen

Description: Cell-permeable non-fluorescent reactive oxygen species (ROS) indicator. Passively diffuses across membranes where it is oxidized by peroxynitrite to rhodamine 123 which localizes in the mitochondria and exhibits green fluorescence (Ex/Em wavelengths of 500 and 536 nm). Used to investigate reactive oxygen intermediates produced by human and murine phagocytes, activated rat mast cells and cultured endothelial cells. In addition, it has been used together with Fura Red calcium indicator to simultaneously measure oxidative bursts and Ca2+ fluxes in monocytes and granulocytes. Dihydrorhodamine 123 has been shown to be a more sensitive probe than H2DCFDA for detecting granulocyte respiratory bursts.

Dihydrorhodamine 123

Catalog Number: (103011-336)

Supplier: Anaspec Inc

Description: Generic substrate for fluorimetric detection of oxidases (including peroxidase) in mitochondria

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Dihydrorhodamine 123 ≥95% (by HPLC), Ultrapure

Catalog Number: (89166-010)

Supplier: Enzo Life Sciences

Description: Mitochondria dye

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Dihydrorhodamine 123 ≥95% (by HPLC), Calbiochem®

Catalog Number: (80051-416)

Supplier: MilliporeSigma

Description: Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite.

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Dihydrorhodamine 123 ≥95%

Supplier: AAT BIOQUEST INC

Description: DihydrorH₂O₂damine 123 is by far the most-used probe for measurement of intracellular H₂O₂

Neutrophil/Monocyte Respiratory Burst Assay Kit, Cayman Chemical Company

Catalog Number: (75817-102)

Supplier: Cayman Chemical Company

Description: Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

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Reactive Oxygen Species Indicators, Biotium

Supplier: Biotium

Description: Commonly used fluorescent dyes for detection of oxidative activities in cells.

MnTBAP

Catalog Number: (80053-226)

Supplier: MilliporeSigma

Description: Cell-permeable superoxide dismutase (SOD) mimetic and peroxynitrite scavenger. Protects endothelial cells in a dose-dependent manner (EC50~40 µM) against paraquat (2 mM). Inhibits the oxidation of Dihydrorhodamine 123 by peroxynitrite, but does not scavenge nitric oxide (NO).

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Dihydrofluorescein diacetate

Supplier: Adipogen

Description: Dihydrofluorescein diacetate is a fluorescent probe for detecting intracellular oxidants. It is hydrolyzed by cellular esterases to dihydrofluorescein and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as Dihydrorhodamine 123 (Ex/Em: 490/514nm).

Dihydrorhodamine 6G

Supplier: TCI America

Description: Dihydrorhodamine 6G

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10-Acetyl-3,7-dihydroxyphenoxazine ≥95% (by HPLC)

Catalog Number: (103011-304)

Supplier: Anaspec Inc

Description: 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex® Red and Ampliflu™ Red, is not only a sensitive and stable fluorogenic substrate for HRP but also an ultrasensitive probe for H2O2. In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, Zhou, et al. have demonstrated that ADHP can be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc. We offer the best quality of ADHP with the most competitive price. The reagent can be purchased in a single 25 mg vial or can be custom-packaged to meet your special requirements.