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You Searched For: 5(6)-Carboxyfluorescein+diacetate+NHS+ester+[CFSE+(5(6)-CFDA+SE)]


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5(6)-Carboxyfluorescein

Supplier: Anaspec Inc
Description: Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.

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BCECF AM ester ≥95% (by HPLC) fluorescent pH indicator

Supplier: Biotium
Description: BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells.

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BCECF AM ester ≥85% (by HPLC), Calbiochem®, Sigma-Aldrich®

Catalog Number: (80050-710)
Supplier: MilliporeSigma
Description: A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubule

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Product Image-76311-696

NHS Ester, SureLight® 488 molecular biology stain

Supplier: VWR International
Description: SureLight® 488 NHS Ester is a bright green fluorescent dye with excitation suited to the 488nm laser line, fluorescent microscopy and flow cytometry. It offers a much more stable signal generation in imaging and flow cytometry than FITC and is half the cost of Alexa Fluor® 488 with no royalties or licensing fees.

SDS

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BCECF AM ester ≥95% (by HPCE) fluorescent pH indicator

Supplier: Adipogen
Description: Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.

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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate N-succinimidyl ester (CDCFDA) ≥96% (by HPLC)

Supplier: Adipogen
Description: 5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Product Image-89139-832

Reactive CF® Dyes and Protein Labeling Kits, Biotium

Supplier: Biotium
Description: Biotium offers a number of reactive dye formats for labelling proteins, nucleic acids, and other biomolecules with fluorescent dyes. CF® dyes offer advantages in brightness and photostability compared other similar commercial dyes. CF® dyes are available in a number of reactive dye formats for CF® dye colours span the visible and near-infrared spectra.

Product Image-76485-216

BCECF AM ester, CytoFix™ Optimized for long term cellular pH tracking

Catalog Number: (76485-216)
Supplier: AAT BIOQUEST INC
Description: Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes.

Small Business Enterprise Minority or Woman-Owned Business Enterprise


Product Image-TS34725-0010

2',7'-Dichlorofluorescein 3',6'-diacetate 97%

Supplier: Thermo Scientific Chemicals
Description: 2',7'-Dichlorofluorescein 3',6'-diacetate 97%

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2',7'-Dichlorofluorescein 3',6'-diacetate

Catalog Number: (103011-330)
Supplier: Anaspec Inc
Description: Cell-permeable substrate for fluorimetric detection of oxidases (including peroxidase)
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2',7'-Dichlorofluorescein 3',6'-diacetate 98%

Supplier: Ambeed
Description: 2',7'-Dichloro-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl diacetate, Purity: 98%, CAS Number: 2044-85-1, Appearance: Form: solid, Storage: Sealed in dry, Room Temperature, Size: 250mg

Image Unavailable-80051-396

2',7'-Dichlorofluorescein 3',6'-diacetate ≥94% (by HPLC), Calbiochem®

Catalog Number: (80051-396)
Supplier: MilliporeSigma
Description: Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (NO) and for the determination of the overall oxidative stress in toxicological phenomena.

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Product Image-89165-934

Calcein AM ≥95% (by HPLC), Ultrapure

Catalog Number: (89165-934)
Supplier: Enzo Life Sciences
Description: Calcium dye
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Calcein AM ≥95% (by HPLC) fluorescent dye

Catalog Number: (103011-396)
Supplier: Anaspec Inc
Description: Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability
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Diethyl-1,4-phenylenediacetate 98%

Supplier: Ambeed
Description: Diethyl 2,2-(1,4-phenylene)diacetate, Purity: 98%, CAS Number: 36076-26-3, Appearance: White to light-yellow powder or crystals, Storage: Sealed in dry, Room Temperature, Size: 1g

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Calcein AM 1 mg/mL in anhydrous DMSO fluorescent dye, for histology

Catalog Number: (89139-472)
Supplier: Biotium
Description: Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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