You Searched For: 2-Mercaptoethanol


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Supplier: VWR
Supplier: VWR
Catalog Number: (80501-154)
Supplier: MilliporeSigma
Description: β-Mercaptoethanol, Molecular Biology Grade a disulfide-reducing agent.

Supplier: Thermo Scientific Chemicals
Description: 2-Mercaptoethanol 99%, pure

Supplier: MP Biomedicals
Description: 2-Merceptoethanol is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. 2-Merceptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis. 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

SDS

Supplier: Thermo Scientific Chemicals
Description: Solubilizes proteins by reducing disulfide linkages
Catalog Number: (TCM0058-25G)
Supplier: TCI America
Description: CAS Number: 60-24-2
MDL Number: MFCD00004890
Molecular Formula: C2H6OS
Molecular Weight: 78.13
Purity/Analysis Method: >98.0% (GC)
Form: Clear Liquid
Boiling point (°C): 158
Melting point (°C): -100
Flash Point (°C): 74
Specific Gravity (20/20): 1.12

Supplier: MP Biomedicals
Description: Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-Mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-Mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: Sigma Aldrich
Description: 25ML CAS 60-24-2

Supplier: MP Biomedicals
Description: β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-Mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-Mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Supplier: MilliporeSigma
Supplier: Sigma Aldrich
Description: 1L CAS 60-24-2

Supplier: VWR
Description: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Supplier: Thermo Scientific Chemicals
Description: Liquid
Catalog Number: (102657-808)
Supplier: American Radiolabeled Chemicals
Description: 2-Mercaptoethanol [1-¹⁴C] 0.1 mCi/ml in sterile water, 50-60 mCi/mmol 1.85-2.22 GBq/mmol

Small Business Enterprise


Supplier: TCI America
Description: CAS Number: 60-24-2
MDL Number: MFCD00004890
Molecular Formula: C2H6OS
Molecular Weight: 78.13
Purity/Analysis Method: >99.0% (GC)
Form: Clear Liquid
Boiling point (°C): 158
Melting point (°C): -100
Flash Point (°C): 74
Specific Gravity (20/20): 1.12
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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