2-Mercaptoethanol ≥98%, liquid for molecular biology
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Danger
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β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
- Molecular Biology Reagent Grade
- Concentration: 14.3 M (pure liquid)
- Vapor Density: 2.69 (air = 1) (Lit.)
- Vapor Pressure: 1 mmHg ( 20 °C) (Lit.)
- Refraxtive Index: n20/D 1.500 (lit.)
- Miscible with water, alcohol, ether and benzene.
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Formula:
HSCH₂CH₂OH Boiling Pt: 154…161 °C (lit.) Melting Pt: < …50 °C (lit.) Density: 1.12 g/cm³ (20 °C) (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00004890 CAS Number: 60-24-2 UN: 2966 ADR: 6.1,II |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥98% |
| APHA Color | ≤10 |
| Water | ≤2000 |
| Distillation 5% | ≥155 |
| Distillation 95% | ≤160 |
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