2-Mercaptoethanol ≥98%, liquid cell culture reagent
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Danger
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Synonyms:
2-Hydroxyethyl mercaptan, β-Mercaptoethanol
Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
- Cell Culture Reagent
- Auto Ignition: 295 °C (Lit.)
- Vapor Density: 2.69 (air = 1) (Lit.)
- Vapor Pressure: 0.76 hPa at 20 °C (Lit.)
- Refractive Index: n20/D 1.500 (Lit.)
- Presentation: Liquid
- Miscible with water, alcohol, ether and benzene.
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Formula:
HSCH₂CH₂OH MW: 78.14 g/mol Boiling Pt: 154…161 °C (lit.) Melting Pt: < …50 °C (lit.) Density: 1.12 g/cm³ (20 °C) (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00004890 CAS Number: 60-24-2 UN: 2966 ADR: 6.1,II |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥98% |
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