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Blocking Buffer For Immunohistochemistry Serum And Azide Free, Rockland Immunochemicals

Catalog Number: (RLMB-071-0100)
Supplier: Rockland Immunochemical
Description: Protein blocker specifically designed for immunohistochemical staining where detection occurs using an immunoenzymatic reaction with substrate, i.e. peroxidase (HRP) with diaminobenzidine (DAB)

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Product Image-89407-062

CulGenex™ Heat-and-Pour™ Agarose Gel Formulations & Reagents, Hardy Diagnostics

Catalog Number: (89407-062)
Supplier: Hardy Diagnostics
Description: CulGenex™ Heat-and-Pour™ Agarose Gels, which contain ethidium bromide, agarose, and with either TAE or TBE buffers, are recommended for agarose gel electrophoresis in the separation, visualization, quantification, or isolation of particular DNA fragments.

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Image Unavailable-100359-102

Phosphate Buffered Saline/Casein Block & Diluent-5X, BioFX®, Surmodics IVD, Inc.

Supplier: Surmodics
Description: BioFX® Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.

Image Unavailable-TCD3647-1G

Dithiothreitol (DTT, Cleland's reagent) ≥98.0% (by titrimetric analysis)

Supplier: TCI America
Description: CAS Number: 3483-12-3
MDL Number: MFCD00004877
Molecular Formula: C4H10O2S2
Molecular Weight: 154.24
Purity/Analysis Method: >98.0% (T)
Form: Crystal
Boiling point (°C): 130
Melting point (°C): 43
Flash Point (°C): 113
Storage Temperature: 0-10°C

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Image Unavailable-IC15123925

1-Heptanesulfonic acid sodium salt, hydrate ~98%, white powder

Catalog Number: (IC15123925)
Supplier: MP Biomedicals
Description: Sodium 1-heptanesulfonate is a commonly used ion pairing reagent for HPLC, including analyses of peptides and proteins. It has also been used in high performance capillary electrophoresis analysis of peptides.

SDS


Product Image-82022-700

FOCUS™ Proteome Kits, G-Biosciences

Supplier: G-Biosciences
Description: An effective proteome analysis requires the preparation of a sample to bring the wide range of protein species into the dynamic range of detection

Product Image-PI17926

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol), white ≥99.8%, crystalline powder, Pierce™

Catalog Number: (PI17926)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Tris is a standard reagent for preparation of many molecular biology buffers.

SDS Certificates


Image Unavailable-76177-956

Glycine ≥99% for molecular biology

Supplier: MP Biomedicals
Description: Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Product Image-IB15720

Ethanol denatured ≥95%, Biotechnology Grade (denatured with 5% methanol)

Catalog Number: (IB15720)
Supplier: IBI Scientific
Description: Ethanol is widely used for precipitating nucleic acids. IBI 200 Proof Ethanol is denatured with 5% methanol.
Product Image-IB07062

Sodium dodecyl sulfate (SDS) ≥99.0%

Catalog Number: (IB07062)
Supplier: IBI Scientific
Description: SDS is an ionic detergent used to denature proteins in hybridization, nucleic purification, and electrophoresis buffer systems. SDS is also used to dissociate nucleic acid protein complexes in DNA extraction protocols, to disrupt cell membranes and to prepare prehybridization and/or hybridization solutions.

SDS


Image Unavailable-76177-672

Acrylamide ≥99%, white crystalline powder for molecular biology

Supplier: MP Biomedicals
Description: Storage: Room Temperature, desiccate.
Acrylamide is prepared on an industrial scale by the hydrolysis of acrylonitrile by nitrile hydratase.
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules.
Acrylamide is an extremely hazardous substance in either the powder form or liquid form. Once polymerized, the polyacrylamide is considered safe to handle under normal laboratory conditions. Acrylic Acid Content -≤0.001%

Product Image-IB70162

TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride) ≥99.5%

Catalog Number: (IB70162)
Supplier: IBI Scientific
Description: Tris-HCl is commonly used in biological and electrophoresis buffer systems and can be used to make a concentrated stock of buffer solutions.

SDS


Product Image-10802-932

Anti-IgG Mouse Monoclonal Antibody (DyLight® 800) [clone: eB182]

Catalog Number: (10802-932)
Supplier: Rockland Immunochemical
Description: Rabbit IgG TrueBlot® is a unique DyLight™ 800 conjugated anti-rabbit IgG immunoblotting (second step) reagent. Rabbit IgG TrueBlot® enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Rabbit IgG TrueBlot®, simply substitute the conventional DL800 anti-rabbit IgG blotting reagent with Fluorescent Rabbit TrueBlot® Antibody DyLight™ 800 and follow the prescribed protocol for sample preparation and immunoblotting. Ideal for Li Cor Odyssey imaging as well as other IR and near IR imaging systems.

Rabbit IgG TrueBlot® is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of rabbit IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Rabbit IgG TrueBlot® with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Fluorescent Rabbit TrueBlot® Antibody DyLight™ 800 may also be used for detection in immunoassays that do not employ immunoprecipitation.
Image Unavailable-76177-742

2-Mercaptoethanol ≥98%, liquid cell culture reagent

Supplier: MP Biomedicals
Description: Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Image Unavailable-76177-226

2-Mercaptoethanol ≥98%, liquid for molecular biology

Supplier: MP Biomedicals
Description: β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

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Image Unavailable-IB40075

Ethidium bromide 10 mg/mL in aqueous solution for molecular biology

Catalog Number: (IB40075)
Supplier: IBI Scientific
Description: Used to detect both single- and double-stranded nucleic acids (both DNA and RNA). Does not intercollate into single-stranded DNA very well; therefore, the fluorescence of single-stranded DNA is relatively poor in comparison to double-stranded DNA. The recommended final concentration of ethidium bromide in agarose gels and electrophoresis buffers is 0.5µg/mL.

SDS


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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