You Searched For: 5,5\'-Dithiobis(2-nitrobenzoic+acid)+(Ellmans+reagent,+DTNB)


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Catalog Number: (80056-346)
Supplier: MilliporeSigma
Description: 5,5''-Dithio-bis-(2-nitrobenzoic Acid). (DTNB; Ellman'' s reagent). Pale yellow solid. PROTECT FROM LIGHT. PROTECT FROM MOISTURE. Sulfhydryl reagent used to characterize reacting SH groups. Useful in photometric determination of thiols.

Catalog Number: (PI22582)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Supplier: Thermo Scientific Chemicals
Description: Reagent for determination of sufhydryl groups
Catalog Number: (102843-490)
Supplier: Matrix Scientific
Description: 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) ≥97%


Catalog Number: (82022-846)
Supplier: G-Biosciences
Description: Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.


Supplier: Enzo Life Sciences
Description: Sensitive sulfhydyl reagent

Catalog Number: (TS11754-0250)
Supplier: Thermo Scientific Chemicals
Description: 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 99%


Supplier: BeanTown Chemical
Description: CAS: 69-78-3; EC No: 200-714-4; MDL No: MFCD00007140; RTECS: DG9650000 Powder; Molecular Formula: C14H8N2O8S2; MW: 396.35 Melting Point: 240-245° (decomposes)

SDS

Supplier: Ambeed
Description: 5,5'-Dithiobis(2-nitrobenzoic acid) (Ellmans reagent, DTNB) 98%

Supplier: Adipogen
Description: A sensitive reagent for measuring the free sulfhydryl content in proteins, peptides, and tissues. Used to characterize reactive thiol groups and photometric determination of thiols and for measuring low-molecular mass thiols such as glutathione in both pure solutions and biological samples, such as blood. It can also measure the number of thiol groups on proteins. Through reaction with aliphatic thiol groups a mixed disulfide of protein thiol and one mole of 2-nitro-5-thiobenzoate per mole of protein sulfhydryl group is being formed. DTNB has little absorbance. Reaction with -SH groups on proteins (from any solvent accessible Cys) under mild alkaline conditions (pH 7-8) produces the 2-nitro-5-thiobenzoate anion, which gives an intense yellow color with an absorption maximum at 409.5nm (Extinction coefficient: 14150 M-1*cm-1). Sensitive to various buffer ions, therefore, the extinction coefficient used to calculate the number of sulfhydryl groups must be matched to the reaction conditions. In case the thiol groups are in disulfide bonds, they must be reduced under anaerobic conditions prior to reaction with DTNB.

Catalog Number: (PI44889)
Supplier: Invitrogen
Description: Thermo Scientific Pierce Cysteine-HCl is purified, crystalline cysteine hydrochloride salt (MW 175.6) that is useful as a sulfhydryl standard in assay with Ellman's Reagent or as an additive in protein refolding experiments

Catalog Number: (82022-782)
Supplier: G-Biosciences
Description: Dithiobis(succinimidyl propionate) Homobifunctional Cross Linker.


Supplier: Ambeed
Description: DSP (Dithiobis(succinimidyl propionate)) 97%

New Product

Supplier: Invitrogen
Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
Supplier: TCI America
Description: [Cross-linking Reagent]
CAS Number: 57757-57-0
MDL Number: MFCD00042045
Molecular Formula: C14H16N2O8S2
Molecular Weight: 404.41
Purity/Analysis Method: >97.0% (N)
Form: Crystal
Melting point (°C): 133
Storage Temperature: 0-10°C

SDS

Catalog Number: (103523-924)
Supplier: Invitrogen
Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

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