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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-102877-564

NEB® PCR Cloning Kit, New England Biolabs

Catalog Number: (102877-564)
Supplier: New England Biolabs (NEB)
Description: This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a linearized vector that uses a novel mechanism for background colony suppression to give a low background.

SDS Small Business Enterprise


Product Image-101417-940

QUICK BLUNTING™ Kit, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The Quick Blunting Kit is used to convert DNA with incompatible 5´ or 3´ overhangs to 5´ phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors

Small Business Enterprise

Product Image-ST200531

Mutagenesis Kits QuikChange, Agilent Technologies

Supplier: Agilent Technologies
Description: The original QuikChange Site-Directed Mutagenesis Kits speed up and simplify site-directed mutagenesis studies. The kits eliminate the need for sub-cloning into M13-based bacteriophage vectors and for ss-DNA rescue. This allows oligo-mediated introduction of site-specific mutations into virtually any double-stranded plasmid DNA. In addition, the XL version of the kit is specially designed for efficient mutagenesis of large (4 -14 kb) or otherwise difficult-to mutagenize plasmid templates. The XL kit features components specifically designed for more efficient DNA replication and bacterial transformation.

SDS

Product Image-99900-316

Gigapack III Packaging Extracts, Agilent Technologies

Supplier: Agilent Technologies
Description: For libraries constructed from highly methylated DNA, Gigapack III Packaging Extract simplifies the packaging procedure and increases the efficiency and representation of libraries constructed from highly methylated DNA. Each packaging extract is restriction minus to optimize packaging efficiency and library representation.

Product Image-76081-604

Ampligase® Thermostable DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: Highly specific thermostable DNA ligase for high-temperature applications

Product Image-97065-204

NxGen™ T4 DNA Ligase, Biosearch Technologies

Supplier: Lucigen
Description: The NxGen® T4 DNA Ligases from LGC Biosearch (Lucigen) come in a range of highly pure grades specifically for next-generation sequencing (NGS). This enzyme catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA and efficiently joins blunt and cohesive ends, and repairs single stranded nicks in duplex DNA, RNA, or DNA/RNA hybrids.

Product Image-103218-906

NEBuilder HiFi DNA Assembly Bundle for Large Fragments, New England Biolabs

Catalog Number: (103218-906)
Supplier: New England Biolabs (NEB)
Description: NEBuilder HiFi DNA Assembly was developed to improve the efficiency and accuracy of DNA assembly

Small Business Enterprise


Product Image-102715-908

T4 RNA Ligase 2 Truncated, KQ, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, truncated KQ (T4 Rnl2tr R55K, K227Q) specifically ligates the preadenylated 5´ end of DNA or RNA to the 3´ OH end of RNA.

Small Business Enterprise

Product Image-PAM1801

T4 DNA Ligase, Promega

Supplier: Promega Corporation
Description: For ligation of DNA ends, mutagenesis and cDNA cloning.

Certificates

Product Image-PAP9801

Bacterial Strain JM109(DE3), Glycerol Stock, Promega

Catalog Number: (PAP9801)
Supplier: Promega Corporation
Description: Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.

Certificates


Product Image-101228-190

T4 RNA Ligase 1 (ssRNA Ligase), New England Biolabs

Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi

Small Business Enterprise

Product Image-101228-194

E. coli DNA Ligase, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: Catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA containing cohesive ends.

Small Business Enterprise

Product Image-PAC8820

Flexi System, Transfer, 100 transfer reactions, Promega

Catalog Number: (PAC8820)
Supplier: Promega Corporation
Description: The Flexi Vector System provides a rapid, efficient, high-fidelity way to transfer protein-coding regions without the need to resequence.

Certificates


Product Image-PAM8225

LigaFast Rapid DNA Ligation System, Promega

Supplier: Promega Corporation
Description: T4 DNA Ligase and buffer combination for rapid (5-minute) ligations.

Certificates

Product Image-102715-942

ELECTROLIGASE®, New England Biolabs

Catalog Number: (102715-942)
Supplier: New England Biolabs (NEB)
Description: ElectroLigase™ combines T4 DNA ligase and an optimized, ready-to-use 2X reaction buffer containing a proprietary ligation enhancer and no PEG.

Small Business Enterprise


Image Unavailable-PAP2301

Bacterial Strain NM522, Promega

Catalog Number: (PAP2301)
Supplier: Promega Corporation
Description: NM522 contains an F' episome, which is required for production of ssDNA and for blue/white color selection.
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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