You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Supplier: New England Biolabs (NEB)
Description: Thermostable 5´ App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum

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Catalog Number: (102715-912)
Supplier: New England Biolabs (NEB)
Description: The Gibson Assembly Cloning Kit combines the power of the Gibson Assembly Master Mix with NEB 5-alpha Competent E.coli, enabling successful assembly of multiple DNA fragments, regardless of fragment length or end compatibility and transformation in just under 2 hours.

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Catalog Number: (102877-596)
Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 1, High Concentration (M0437M), is a 3X higher concentration than our standard T4 RNA Ligase 1 (M0204S/L)

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Supplier: New England Biolabs (NEB)
Description: Gibson Assembly was developed by Dr

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Catalog Number: (PAC1263)
Supplier: Promega Corporation
Description: For ligation of DNA ends.

Supplier: New England Biolabs (NEB)
Description: The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature
Supplier: New England Biolabs (NEB)
Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), has both intermolecular and intramolecular RNA strand joining activity

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Supplier: New England Biolabs (NEB)
Description: NEBridge Ligase Master Mix is a 3X master mix for Golden Gate Assembly. Designed for use with NEB Type IIS restriction enzymes, this master mix contains T4 DNA Ligase in an optimized reaction buffer with a proprietary ligation enhancer.

Supplier: New England Biolabs (NEB)
Description: SplintR Ligase, also known as PBCV-1 DNA Ligase or Chorella virus DNA Ligase, efficiently catalyzes the ligation of adjacent, single-stranded DNA oligonucleotides splinted by a complementary RNA strand.

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Supplier: New England Biolabs (NEB)
Description: T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3.

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Supplier: New England Biolabs (NEB)
Description: T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7.

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Catalog Number: (102855-130)
Supplier: New England Biolabs (NEB)
Description: The NEBNext® Ultra™ Ligation Module is designed for use with the NEBNext Singleplex or Multiplex Oligos for Illumina.

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Catalog Number: (PAM1051)
Supplier: Promega Corporation
Description: T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Supplier: ReVacc Scientific
Description: This Seamless Assembly Master Mix (2X) uses Gibson cloning principle to allow efficiently assembly of multiple DNA fragments, regardless of fragment length or end compatibility. The insert and the linearized vector need the overlapped sequences of 15 bp - 20 bp on both 5' - and 3' -end, respectively. A positive control with the mixture of insert and linearized vector is included.

Supplier: New England Biolabs (NEB)
Description: Taq DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini of two adjacent oligonucleotides which are hybridized to a complementary target DNA

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Supplier: INTACT GENOMICS, INC MS
Description: T4 DNA ligase displays significantly higher ligation efficiency than the nearest competitor.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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