Bacterial Strain JM109(DE3), Glycerol Stock, Promega

Supplier: Promega Corporation

P9801
PAP9801EA 68.55 USD
PAP9801
Bacterial Strain JM109(DE3), Glycerol Stock, Promega
Nucleic Acid Reagents Cloning Kits and Ligation

Used for high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, if the sequence contains a ribosomal binding site. No blue/white selection.


  • For High-Level Gene Expression from the T7 Promoter
  • Contains an IPTG-inducible gene for T7 RNA polymerase
  • Requires a ribosomal binding site with cloned sequence
  • Lacks blue/white cloning selection


Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection. Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, lambda-, Delta(lac-proAB), [F', traD36, proAB, lacI(q)ZDeltaM15], lDE3.


Precaución: Storage Conditions: Less than -65°C

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