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Catalog Number: (103523-926)

Supplier: Invitrogen

Description: Thermo Scientific Pierce SMCC is a hetero-bifunctional crosslinker that contain N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules. NHS esters react with primary amines at pH 7–9 to form amide bonds, while maleimides react with sulfhydryl groups at pH 6.5–7.5 to form stable thioether bonds. In aqueous solutions, NHS ester hydrolytic degradation is a competing reaction whose rate increases with pH. The maleimide group is more stable than the NHS-ester group, but will slowly hydrolyze and lose its reaction specificity for sulfhydryls at pH values > 7.5. For these reasons, conjugations with these crosslinkers are usually performed at pH 7.2–7.5, with the NHS ester (amine-targeted) reacted before or simultaneous with the maleimide (sulfhydryl-targeted) reaction.

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Catalog Number: (103523-944)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-SANPAH is a hetero-bifunctional crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

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Catalog Number: (PI88378)

Supplier: Invitrogen

Description: Thermo Scientific™ 96-Well Plates are manufactured using high-quality polystyrene and are high-binding with black, flat well bottoms to accommodate fluorescence assays such as the Pierce™ Quantitative Fluorometric Peptide Assay.

Catalog Number: (PI23295)

Supplier: Invitrogen

Description: Thermo Scientific™ Peptide Digest Assay Standard is a validated protein digest reference sample optimized for use in quantitative peptide assays to improve reproducibility and accuracy.Robust peptide assay standard—the only commercially available peptide digest standard that has been developed for use in a quantitative peptide assayValidated—validated for both the Pierce Quantitative Fluorometric Peptide Assay and the Pierce Quantitative Colorimetric Peptide Assay with comparable standard curves using both assaysStable—liquid formulation is stored at 4⁰C, eliminating variability due to freeze-thawsEasy-to-use—provided in a liquid format with a known peptide concentrationThe Peptide Digest Assay Standard was designed as reference standard for use with the Pierce Quantitative Fluorometric and Colorimetric Peptide Assays to augment the reproducibility and accuracy of peptide quantitation in a microplate-based assay.The reference protein has been digested with MS-grade trypsin to minimize missed cleavages

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Catalog Number: (PI88341)

Supplier: Invitrogen

Description: The Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade, is a validated protein digest optimized for use as a quality control sample for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples.Positive control sample—BSA digest optimized and validated as a quality control standard for mass spectrometry applicationsExcellent sequence coverage—greater than 85% sequence coverageValidated peptide quality—digestion procedure optimized for minimal missed cleavages and over-alkylationRigorously tested—high-quality, consistent BSA protein digest documented via lot-specific certificates of analysisStable—provided in a stable lyophilized formatThe Pierce BSA Protein Digest Standard is produced using high quality bovine serum albumin (BSA) and mass spectrometry-grade trypsin. The BSA is fully reduced, alkylated (iodoacetamide), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard that is free of intact protein. The peptide digest standard provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest standard has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.

SDS Certificates

Supplier: Invitrogen

Description: Thermo Scientific SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for picogram to high-femtogram level detection.

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Supplier: Invitrogen

Description: Hydrophobic transfer membranes made of high quality PVDF, which provides high binding capacity for proteins and nucleic acids.

Catalog Number: (PIKIT036)

Supplier: Invitrogen

Description: Assay NGAL (human neutrophil gelatinase-associated lipocalin) levels in human serum, plasma (EDTA, heparin, sodium citrate), tissue extracts, urine, and culture supernatant samples with our Thermo Scientific Human NGAL ELISA Kit.

Supplier: Invitrogen

Description: Thermo Scientific Imject Maleimide-Activated Blue Carrier Protein enables conjugation of sulfhydryl-containing peptide antigens to this soluble immunogenic carrier protein for immunization and effective antibody production.

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Supplier: Invitrogen

Description: Pierce™ Imject™ Blue Carrier™ protein is a highly soluble, mollusc-derived hemocyanin that enable simple preparation of effective immunogens with amine- or carboxyl-peptide antigens.

Supplier: Invitrogen

Description: The Imject™ EDC Carrier Protein Spin Kits enable effective one-step conjugation of a hapten to a carrier protein using EDC as the crosslinker. The resulting conjugate is used for eliciting an immune response and antibody production against the hapten.

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Catalog Number: (PI16138)

Supplier: Invitrogen

Description: Thermo Scientific EZ-Link™ Desthiobiotinylation and Pull-down Kit is a complete set of reagents to biotin-label purified proteins and use them to purify interaction complexes using streptavidin agarose resin with mild elution conditions.

Supplier: Invitrogen

Description: Thermo Scientific Pierce HisPur™ Ni-NTA Superflow Agarose is a nitrilotriacetic acid modified Superflow 6 support charged with divalent nickel (Ni⁺²) used for FPLC purification of poly-histidine-tagged proteins.

SDS Certificates

Supplier: Invitrogen

Description: Single-Use RED (rapid equilibrium dialysis) Plate is composed of disposable high-density polypropylene and is pre-loaded with 48 equilibrium dialysis membrane inserts. Each insert is comprised of two side-by-side chambers separated by an O-ring-sealed vertical cylinder of dialysis membrane with varying molecular-weight cutoffs (8 or 12 kD MWCO).

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Supplier: Invitrogen

Description: Pierce™ HisPur™ Cobalt Purification Kit is designed for the efficient purification of recombinant polyhistidine-tagged proteins from bacterial, mammalian and baculovirus-infected cells.

SDS Certificates

Catalog Number: (PI89971)

Supplier: Invitrogen

Description: Pierce, Cartridge accessory pack, includes plastic fittings for chromatography cartridges