You Searched For: MP BIOMEDICALS (FKA ICN BIOMED

Supplier: MP Biomedicals

Description: RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent.

Supplier: MP Biomedicals

Description: Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Catalog Number: (IC091642954)

Supplier: MP Biomedicals

Description: Dulbecco's Modification of Eagle's Medium 1X with 4.5 g/l glucose, without L-glutamine, i-inositol is ideally suited for supporting and maintaining a vast range of mammalian cell types.

Catalog Number: (75877-464)

Supplier: MP Biomedicals

Description: Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice. Anti-Mouse IgG (whole molecule)-FITC antibody reacts with normal mouse serum and all mouse IgGs.

Fluorescein-Conjugated Goat Affinity Purified Antibody to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.

The total protein is measured by absorbance (A280 nm); F/P ratio is calculated using absorbance at 491.5 nm. Antibody titer of the affinity purified antibody is standardized by equivalence-point precipitation. The affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.

Catalog Number: (76202-278)

Supplier: MP Biomedicals

Description: Superbroth is used for obtaining high yields of lambda phage and phagemids in <i>E. coli</i>.

Catalog Number: (76202-284)

Supplier: MP Biomedicals

Description: 2XYT-Broth is a nutritional rich medium providing nitrogen and growth factors optimized for the growth of recombinant <i>E. coli</i> strains infected with the M13 bacteriophage or other filamentous single stranded DNA bacteriophages thus allowing the bacteriophage to reproduce in large quantities without exhausting the host cells.

Catalog Number: (76177-058)

Supplier: MP Biomedicals

Description: 2,2-Azino-bis-(3-ethyl-benzthiazoline-6-sulfonic acid) (ABTS) has been used as a chromogenic substrate for horseradish peroxidase (HRP), both in general activity assays and in ELISA applications. Activity of HRP using ABTS appears about four-fold higher than using pyrogallol. It is mainly used as a substrate in sensitive peroxidase assays.
2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.

Catalog Number: (IC0855914)

Supplier: MP Biomedicals

Description: Purified human IgM is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Catalog Number: (76461-488)

Supplier: MP Biomedicals

Description: Tris(hydroxymethyl)aminomethane (TRIS, Trometamol)

Catalog Number: (75877-358)

Supplier: MP Biomedicals

Description: Product is the lyophilized powder of goat antiserum to human secretory IgA and buffer salts.

IgA antibody is a secretory antibody found abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. Secretory IgA contributes to the humoral defense mechanism against the pathogens on mucosal surfaces. Anti-Human IgA (α-chain specific) antibody specifically recognizes human IgA. Immunoglobulin A (IgA) is the primary antibody involved in mucosal immunity. It is identified by the presence of α-type heavy chains. IgA, which is composed of two subclasses IgA1 and IgA2, can exist in a dimeric secretory form found in mucous secretions.

The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence-point precipitation. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.

The antiserum is prepared by hyperimmunizing goats with highly purified human secretory IgA; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is suitable for Ouchterlony double diffusion. The antibody was used for IgA quantification by ELISA in various studies. Antibody dilution of 1:4000 was used for passive hemagglutination inhibition assay.

Supplier: MP Biomedicals

Description: Dimethyl Sulfoxide is ideal for use as a cryoprotectant. Also used in chemical reactions, in polymerase chain reactions (PCR) as a PCR cosolvent to help improve yields, especially in long PCR, and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal-induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines, embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). Used to enhance dermal absorption of many chemicals. A solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers. Used in antifreeze or hydraulic fluids. A cryopreservative for cell cultures. Used in the oxidation of thiols and disulfides to sulfonic acids.

Dimethyl sulfoxide (DMSO) is a highly polar aprotic organic reagent that has exceptional solvent properties for organic and inorganic chemicals

Physical Apppearance: Liquid
Viscosity: 1.1 centipoises (27 °C)
Vapor Density: 2.7 (vs air)
Vapor Pressure: 0.55 hPa at 20 °C, 0.42 mmHg (20 °C)
Refractive Index: n20/D 1.479(lit.)
Solubility: Soluble in water (1 mL DMSO + 1 mL H2O), methanol, acetone, ether, benzene, chloroform

Supplier: MP Biomedicals

Description: Sodium Perchlorate Monohydrate is used in standard DNA extraction and hybridization reactions in molecular biology.
Physical Appearance: White Crystalline Powder
pH: 6.0 - 8.0 (5% aq soln)
Solubility: Very soluble in water

Supplier: MP Biomedicals

Description: Collagenases degrade native helical collagen fibrils

Certificates

Catalog Number: (76020-266)

Supplier: MP Biomedicals

Description: Sterile YT media formulations are optimized for the growth and maintenance of M13 phage or other filamentous ssDNA bacteriophages.

Supplier: MP Biomedicals

Description: Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

Supplier: MP Biomedicals

Description: Indocyanine green (ICG) is a cyanine dye used in medical diagnostics.