You Searched For: Western Blot Detection Systems

Supplier: GVS FILTER TECHNOLOGY

Description: Supported Nitrocellulose (MCE) membranes.

Catalog Number: (76235-518)

Supplier: Rockland Immunochemical

Description: AKT1 Western Bloting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Catalog Number: (76235-516)

Supplier: Rockland Immunochemical

Description: AKT3 Western Bloting kit allows for the detection of endogenous protein levels of human AKT3 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT3. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT3 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT3 Western Bloting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Catalog Number: (89155-884)

Supplier: Enzo Life Sciences

Description: This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites.

Supplier: Vector Laboratories

Description: This VECTASTAIN® Elite® ABC system is the most sensitive avidin/biotin-based peroxidase system and is particularly important in the localization of antigens present in low amounts or in cases where the cost of the primary antibodies is significant.

Supplier: Biotium

Description: A high quality biotin-binding protein conjugated to Biotium's signature bright and photostable fluorescent CF® dyes, and a selection of other labels.

Supplier: Li-Cor

Description: REVERT total protein stain is a membrane stain that fluoresces at 700 nm. This does not covalently modify sample proteins and therefore does not affect antibody binding or quantification. Total protein stain works with existing western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes and is compatible with both PVDF and nitrocellulose membranes.

SDS

Supplier: Cell Biolabs

Description: PhosphoBLOCKER™ Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio

Catalog Number: (AAJ60461-AP)

Supplier: Thermo Scientific Chemicals

Description: Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result

SDS Certificates

Catalog Number: (82021-410)

Supplier: G-Biosciences

Description: BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

Catalog Number: (RLFEMTOMAX-020)

Supplier: Rockland Immunochemical

Description: Chemiluminescent Femtomax™ Super Sensitive HRP Substrate For Microwell And/Or Membrane (2 Component System)

Supplier: Vector Laboratories

Description: VECTASTAIN® ABC kits take advantage of the flexibility and stability of avidin/biotin binding for sensitive, cost-effective chromogenic target protein detection on tissue slices and blots.

Catalog Number: (RL000001J08)

Supplier: Rockland Immunochemical

Description: E.coli HCP Control Peptide

Catalog Number: (95040-078)

Supplier: Cytiva

Description: Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560 nm when excited at the appropriate wavelength.

SDS Certificates

Supplier: MP Biomedicals

Description: A chromogenic substrate for alkaline phosphatase.

SDS

Supplier: Cytiva

Description: Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.

Certificates