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Supplier: Quantabio
Description: qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5’-hydrolysis probes on real-time PCR systems that do not require a passive reference dye. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. qScript XLT One-Step RT-qPCR ToughMix contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.
Catalog Number: (PAA7170)
Supplier: Promega Corporation
Description: A 15-minute column purification method for purifying PCR-amplified DNA away from contaminants, including primer-dimers and amplification primers. Can also be used to purify DNA fragments from agarose gels.

Supplier: INTACT GENOMICS, INC MS
Description: i7™ Hhot start high-fidelity DNA polymerase 2X master mix is ready to use premix which contains hot start high-fidelity DNA polymerase, dNTPs,MgCl₂, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7™ hot start high-fidelity DNA polymerase 2X master mix has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes.

Supplier: MP Biomedicals
Description: Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Supplier: Cytiva
Description: illustra™ ExoProStar™ contains illustra™ Alkaline Phosphatase and Exonuclease 1, formulated to work together to remove unincorporated primers and nucleotides from amplification reactions in preparation for sequencing, cloning, genotyping or further DNA modification reactions.
Catalog Number: (PAA7710)
Supplier: Promega Corporation
Description: System for purification of single-stranded DNA from whole blood or blood stains. DNA may be used directly in amplification reactions without further manipulation. No organic extraction or ethanol precipitation.

Supplier: Omega Bio-Tek
Description: Gel purification of DNA is a common technique used to isolate specific DNA fragments following agarose gel electrophoresis

SDS Minority or Woman-Owned Business Enterprise

Catalog Number: (10147-142)
Supplier: Omega Bio-Tek
Description: The Mag-Bind® RxnPure Plus Kit allows rapid and reliable isolation DNA from PCR and other enzymatic reactions with high yield.

Supplier: Omega Bio-Tek
Description: The Endo-Free Plasmid Midi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA
Supplier: Omega Bio-Tek
Description: The Endo-Free Plasmid Maxi Kits provide an efficient method for the large-scale isolation of endotoxin-free plasmid DNA
Supplier: Zymo Research
Description: The EZ DNA Methylation™ Kits feature simplified procedures that streamline bisulfite treatment of DNA.

Supplier: Corning
Description: The AxyPrep™ Mag Tissue gDNA Kit utilizes a magnetic bead-based technology to isolate genomic DNA from rodent tail tissues. DNA from other tissue types may be extracted with this protocol, but optimisation may be required.
Catalog Number: (PAN1301)
Supplier: Promega Corporation
Description: The MethylEdge Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours.

Supplier: Corning
Description: AxyPrep™ Mag plasmid purification Kit utilizes a magnetic bead technology for high throughput purification of plasmid DNA from <i>E.coli</i> cells. The AxyPrep™ Mag kit can also be used with fosmid and BAC vector-based constructs ranging from 5 to 150 Kb. The system consists of steps of pelleting cells, resuspension, lysis, neutralization, DNA binding, ethanol washing and plasmid elution. The protocol is automation-compatible.
Supplier: VWR International
Description: An optimal PCR buffer system is critical for the performance of successful PCR.

Supplier: VWR International
Description: VWR® Taq DNA polymerase is an ultra-pure, thermostable, recombinant DNA polymerase, which provides robust PCR performance in a wide range of PCR applications, without time-consuming optimization.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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