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NEBNext® Q5U® Master Mix, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBNext® Q5U® master mix contains a modified version of Q5® High Fidelity DNA Polymerase, optimized for amplification of uracil-containing NGS libraries. This hot start formulation is designed to read through uracils, providing robust, high-fidelity and uniform library amplification.

Product Image-ST600679

Herculase II Fusion DNA Polymerases, Agilent Technologies

Supplier: Agilent Technologies
Description: Herculase II Fusion DNA Polymerases produce superior yields with extension times as short as 15 sec/kb, even with genomic DNA targets up to 23kb. Herculase II helps overcome PCR challenges with successful amplification of targets of all complexity. Easily amplify low abundance, high GC, and other difficult targets with robust yields.

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Product Image-95042-266

illustra GenomiPhi V2 DNA Amplification Kits, Cytiva

Supplier: Cytiva
Description: illustra™ GenomiPhi™ V2 DNA Amplification Kit, part of the Phi29 DNA polymerase family, is for mini-scale genomic DNA preparation. The protocol is simple. A typical DNA yield of 4 to 7 µg DNA can be achieved in less than two hours with limited hands-on time. The average product length is >10 kb. The starting material for GenomiPhi reactions can be purified DNA from any commercial kit or homebrew method, or non purified cell lysate.

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Product Image-PAM4021

GoTaq Long PCR Master Mix, 100 reactions, Promega

Catalog Number: (PAM4021)
Supplier: Promega Corporation
Description: GoTaq Long PCR Master Mix contains the high-performance GoTaq Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase.

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Product Image-95040-350

illustra GenomiPhi HY DNA Amplification Kits, Cytiva

Supplier: Cytiva
Description: The illustra™ GenomiPhi™ HY DNA Amplification Kit is part of the Phi29 DNA polymerase family of products. It contains all of the components necessary for genomic DNA preparation by isothermal strand displacement amplification. The product protocol is very simple, and provides typical yields of 40 to 50 μg DNA and average product lengths 10 kb. The starting material for GenomiPhi reactions can be purified DNA or non purified cell lysates.

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Product Image-76485-404

ReadiUse™ dNTP Mix *10 mM* (5 ml), AAT Bioquest

Catalog Number: (76485-404)
Supplier: AAT BIOQUEST INC
Description: Our Ready-to Use Solution of deoxynucleotide triphosphates (dNTPs) have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labeling, DNA sequencing, in vitro transcription, siRNA synthesis, and RNA amplification.

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CYTAG® Total CGH Labeling Kit, Enzo Life Sciences

Supplier: Enzo Life Sciences
Description: Complete kit to digest and label DNA for CGH+SNP arrays.

Product Image-10002-238

FastPROBE™ qPCR Lo-ROX Master Mix, Tonbo Biosciences

Supplier: Tonbo Biosciences
Description: FastPROBE™ qPCR Lo-ROX Master Mix is ready-to-use cocktail for the amplification and quantitation of DNA on real-time instruments that support normalization with a standard ROX passive reference dye at a low final concentration of 50 nM.

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PHU-50 High Fidelity Polymerase

Supplier: EMPIRICAL BIOSCIENCE
Description: Phu-50 High Fidelity Polymerase is the ideal choice for applications where the efficient amplification of DNA with highest fidelity is required. The enzyme is a genetically engineered Phu DNA polymerase but showing a 2-fold higher accuracy and an increased processivity, resulting in shorter elongation times. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in 5 - 3' direction but does not possess a 5 - 3' exonuclease replacement activity. Its inherent 3 - 5' exonuclease proofreading activity results in a greatly increased fidelity of DNA synthesis compared to Taq polymerase. Phu-50 Polymerase-generated PCR fragments are blunt.

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Product Image-89134-006

illustra TempliPhi™ DNA Sequencing Template Amplification Kit

Catalog Number: (89134-006)
Supplier: Cytiva
Description: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

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Product Image-PAM5001

GoTaq Hot Start Polymerase, Promega

Supplier: Promega Corporation
Description: GoTaq Hot Start Polymerase contains the high-performance GoTaq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two minutes.

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Product Image-89131-612

illustra™ TempliPhi™100/500 Amplification Kits, Cytiva

Catalog Number: (89131-612)
Supplier: Cytiva
Description: TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.

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Product Image-76284-672

NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 2), New England Biolabs

Catalog Number: (76284-672)
Supplier: New England Biolabs (NEB)
Description: NEBNext Multiplex Oligos provide adaptors and primers to enable high yield multiplex Illumina library production. The unique hairpin loop structure of the NEBNext Adaptor minimizes adaptor-dimer formation, and NEBNext index PCR primers enable index incorporation during library amplification.
Product Image-PAD6006

GoTaq MDx Hot Start Polymerase, Promega

Supplier: Promega Corporation
Description: GoTaq MDx Hot Start Polymerase contains GoTaq MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.

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Product Image-76423-470

EvaGreen® Plus Dye, 20X in Water, Biotium

Supplier: Biotium
Description: EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Product Image-10002-228

CYBRFast™ qPCR Hi-ROX Master Mix, Tonbo Biosciences

Supplier: Tonbo Biosciences
Description: CYBRFast qPCR Hi-ROX Master Mix is a ready-to-use cocktail for the amplification and detection of DNA on real-time instruments that support normalization with a standard ROX passive reference dye at a final concentration of 500 nM

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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