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Water, Cell Culture Grade low in endotoxins

Supplier: VWR

Description: VWR® Cell culture grade water is extremely low in endotoxin, free of DEPC (Diethyl pyrocarbonate) and other chemical additives. The product is 0.2 um filtered. It is suitable in cell culture applications.

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Phosphate Buffered Saline (PBS),Powdered, Ultra Pure Grade

Supplier: VWR

Description: 1X Solution Composition:137 mM NaCl,2.7 mM KCl,9.8 mM Phosphate buffer
May be sterilized by filtration.
Does not contain magnesium or calcium.
pH at 25°C (1X PBS): 7.3 – 7.5
Conductivity at 25°C (1X PBS): 13,500-17,000 umhos

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Imidazole ≥99% for biotechnology

Supplier: VWR

Description: Biological buffers are useful for cell culture in vitro, enzyme assays and some electrophoretic applications at physiological pH. Universally applicable buffers for biochemistry must be water soluble, not interfere with biological processes or biological

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HEPES Buffered Saline, Promocell

Supplier: Promocell

Description: These solutions are prepared from raw materials of the highest quality using tissue culture grade water.

Bis(2-hydroxyethyl)amino tris(hydroxymethyl)methane (BIS-TRIS), Ultrapure

Supplier: VWR

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1.0 M HEPES pH 7.2

Supplier: Rockland Immunochemical

Description: HEPES is a zwitterionic organic chemical buffering agent.

Water, UltraPure for molecular biology, sterile filtered

Supplier: Rockland Immunochemical

Description: Molecular Biology Grade Ultrapure water is suitable for molecular immunology, molecular biology and nucleic acid methodologies, when applicable. It is commonly used in laboratory to prepare buffers in biochemistry and molecular biology laboratories and also cell culture media for cell biology studies.

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium, MP Biomedicals

Catalog Number: (IC091960454)

Supplier: MP Biomedicals

Description: Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Sodium hydroxide 1 N

Catalog Number: (95016-356)

Supplier: HiMedia

Description: Sodium hydroxide (1 N) solution prepared in cell culture grade water. It is used to buffer cell culture medium as per application.

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MOPSO (3-(4-morpholino)-2-hydroxypropanesulfonic acid) free acid, Ultra Pure Grade

Catalog Number: (97064-266)

Supplier: VWR

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VWR® Sodium Phosphate 0.1 M, pH 7.0 Buffer

Catalog Number: (BDH7895-4)

Supplier: VWR International

Description: Made with sodium phosphate monobasic monohydrate, sodium hydroxide solution and deionized water.

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Water, Molecular Biology Grade

Catalog Number: (VWRL0201-1000)

Supplier: VWR

Description: VWR® Molecular Biology Grade Water is 0.2 um filtered, free of DEPC (Diethyl pyrocarbonate) and other chemical additives. The product is extremely low in endotoxin and has been analyzed for the absence of DNase, RNase and Protease. It is suitable in molecular biology research applications.

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2-Mercaptoethanol ≥98%, liquid cell culture reagent

Supplier: MP Biomedicals

Description: Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.