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Dimethyl sulfoxide for molecular biology

Supplier: MP Biomedicals
Description: Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.

Image Unavailable-102999-804

Human Beta-Amyloid (1-40)

Supplier: Anaspec Inc
Description: Scrambled control peptide of beta-amyloid are often used in studies to compare the effects of wild type Beta-Amyloid (1-40).

Product Image-PAV4107

ADP-Glo Kinase Assay + DNA-PK Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV4107)
Supplier: Promega Corporation
Description: Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ~460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.

SDS


Product Image-PAM9004

AMV Reverse Transcriptase

Catalog Number: (PAM9004)
Supplier: Promega Corporation
Description: AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

Certificates


Image Unavailable-75794-030

Human Recombinant BCMA (from HEK293 cells)

Catalog Number: (75794-030)
Supplier: Prosci
Description: BCMA is a receptor for BAFF and APRIL. It promotes B cell survival and plays a role in the regulation of humoral immunity. It's downstream signaling is dependent on NF-kappaB and JNK.
Image Unavailable-75789-382

Human Recombinant Biglycan (from Cells)

Catalog Number: (75789-382)
Supplier: Prosci
Description: Biglycan is a 200-350 kD proteoglycan consisting of a 45 kD core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains. Biglycan binds to TGF-ß. It also binds to collagen type I in low ionic strength (less than 3 mM phosphate) buffer. At higher ionic strengths, Biglycan does not bind to collagen type I. It enhances the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/ml. It also prevents the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/ml.
Product Image-PAM2206

DNA Polymerase I Large (Klenow) Fragment, Promega

Supplier: Promega Corporation
Description: A DNA-dependent DNA polymerase that lacks the 5'-->3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'-->3' polymerase, 3'-->5' exonuclease and strand displacement activities.

Certificates

Product Image-PAM3011

Single-Stranded DNA, DNA Binding Protein, Promega

Catalog Number: (PAM3011)
Supplier: Promega Corporation
Description: Single-Stranded DNA Binding Protein binds with high affinity to single-stranded DNA but does not bind well to double-stranded DNA.

Certificates


Product Image-102969-102

NEBNext® Ultra II End Repair/dA-Tailing Module, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: The NEBNext Ultra II End Repair/dA-Tailing Module has been optimized to convert 500 pg-1 μg of fragmented DNA to repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends.

Small Business Enterprise

Product Image-10049-530

Anti-HSP91 Rat Monoclonal Antibody

Supplier: Enzo Life Sciences
Description: The 90kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90-alpha are 85% and 90% homologous to those of Hsp90β , respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophila Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits even after Hsp90 function is restored.

Product Image-10049-510

Anti-HSP90 Mouse Monoclonal Antibody [clone: 2D11B9]

Catalog Number: (10049-510)
Supplier: Enzo Life Sciences
Description: The 90kDa molecular chaperone family includes 90kDa heat shock protein Hsp90 and 94kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90α are 85% and 90% homologous to those of Hsp90β, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signalling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophilia Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits, even after Hsp90 function is restored.
Image Unavailable-75842-422

Anti-TLR9 Rat Monoclonal Antibody (PE (Phycoerythrin)) [clone: 72-1665]

Catalog Number: (75842-422)
Supplier: BIOGEMS INTERNATIONAL INC.
Description: The 72-1665 monoclonal antibody specifically reacts with CD289, also known as human toll-like receptor 9 (TLR9). CD289 is involved in the activation of innate immunity, in acquired immune responses, and autoimmune diseases. It is involved in the immune system’s response to unmethylated CpG dinucleotide sequences such as those found in bacterial, viral, or synthetic DNA. CD289 assists in pathogen recognition and is expressed by subtypes of dendritic cells and B cells.
Image Unavailable-75794-272

Human Recombinant CD272 (from CHO Cells)

Catalog Number: (75794-272)
Supplier: Prosci
Description: BTLA is a type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V-type Ig-like domain, which interacts with HVEM.
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Anti-TLR9 Rat Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: M9.D6]

Catalog Number: (75843-288)
Supplier: BIOGEMS INTERNATIONAL INC.
Description: The M9.D6 monoclonal antibody specifically reacts with mouse CD289 also known as toll-like receptor 9 (TLR9). CD289 is involved in the activation of innate immunity, in acquired immune responses, and autoimmune diseases. It is involved in the immune system’s response to unmethylated CpG dinucleotide sequences such as those found in bacterial, viral, or synthetic DNA. CD289 assists in pathogen recognition and is expressed by subtypes of dendritic cells and B cells.
Image Unavailable-75794-274

Human Recombinant CD272 (from CHO Cells)

Catalog Number: (75794-274)
Supplier: Prosci
Description: BTLA is a type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V-type Ig-like domain, which interacts with HVEM.
Image Unavailable-75794-116

Human Recombinant Flagellin (from E. coli)

Catalog Number: (75794-116)
Supplier: Prosci
Description: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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