Dimethyl sulfoxide for molecular biology
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Storage: Room Temperature, desiccate, store under nitrogen.
Dimethyl Sulfoxide is a dipola, aprotic solvent. It has been shown to accelerate strand renaturation (1-10% concentration) and is believed to give the nucleic acid thermal stability against depurination.
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly (A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane.
- Molecular Biology Reagent Grade
- Meets ACS specifications
- Auto Ignition: 573 °F (Lit.)
- Vapor Density: 2.7 (vs air) (Lit.)
- Vapor Pressure: 0.42 mmHg ( 20 °C) (Lit.)
- Refractive Index: n20D 1.479 (lit.)
- Presentation: Liquid
- Dielectric Constant: 45 (Lit.)
- Soluble in water, methanol, acetone, ether, benzene, chloroform.
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Formula:
(CH₃)₂SO Boiling Pt: 189 °C (1013 hPa) (lit.) Melting Pt: 16 …19 °C (lit.) Density: 1.104 g/cm³ (20 °C) (lit.) Storage Temperature: Ambient |
MDL Number:
MFCD00002089 CAS Number: 67-68-5 |
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Specification Test Results
| Assay | ≥99.9% |
| Residue after Evaporation | ≤0.01% |
| Titratable Acid | ≤0.001meq/g |
| Water | ≤0.1% |
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