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Product Image-89407-062

CulGenex™ Heat-and-Pour™ Agarose Gel Formulations & Reagents, Hardy Diagnostics

Catalog Number: (89407-062)
Supplier: Hardy Diagnostics
Description: CulGenex™ Heat-and-Pour™ Agarose Gels, which contain ethidium bromide, agarose, and with either TAE or TBE buffers, are recommended for agarose gel electrophoresis in the separation, visualization, quantification, or isolation of particular DNA fragments.

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Product Image-76510-660

Bis-Tris Mini Protein Gels, GenScript®

Supplier: Genscript
Description: GenScript's Bis-Tris gels are high-performing polyacrylamide gels designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. Furthermore, the neutral pH which minimizes protein modification when compared to Tris-glycine gels.

Product Image-KT420160-0000

Kimble® KONTES® DISPOSAFLEX® Disposable Columns, DWK Life Sciences

Catalog Number: (KT420160-0000)
Supplier: DWK Life Sciences (KIMBLE)
Description: An inexpensive, multi-purpose column designed to satisfy a wide range of analytical separations using aqueous buffers. A typical application of the Disposaflex® column is the extraction of the cyclic nucleotides cAMP and cGMP from sample preparations using AG1-X8.
Image Unavailable-77507-884

Vacuum Filtration Systems

Supplier: NEST SCIENTIFIC
Description: Nest disposable vacuum filtration system is useful for large volume sample separation and sterilization for tissue culture media and other biological buffers by using a vacuum pump to provide differential pressure for the filtration.

New Product

Image Unavailable-76177-412

Orthoboric acid

Supplier: MP Biomedicals
Description: Boric Acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates, leather, carpets, hats, soaps, artificial gems; in nickeling baths; cosmetics; printing and dyeing, painting; photography; for impregnating wicks; electric condensers; hardening steel. Also used as insecticide for cockroaches and black carpet beetles. In the research labs it is used as an astringent, antiseptic, antibacterial and antifungal agent. Used in buffers and for calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis.
Room Temperature, desiccate

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Product Image-29300-124

Ultrafiltration Membrane Disc Filters

Supplier: Cytiva
Description: Replacement membranes for conventional, nondisposable stirred cell systems.

Product Image-IC191411.1

Orthoboric acid ≥99.5%, powder ACS

Supplier: MP Biomedicals
Description: Boric acid is used for weatherproofing wood and fireproofing fabrics; as a preservative; manufacturing of cements, crockery, porcelain, enamels, glass, borates, leather, carpets, hats, soaps, artificial gems; in nickeling baths; cosmetics; printing and dyeing, painting; photography; for impregnating wicks; electric condensers; hardening steel. It is also used as an insecticide for cockroaches and black carpet beetles. It is used In the research labs , astringent, antiseptic, antibacterial and antifungal agent. It is used in buffers and for calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis.

Image Unavailable-IC04814326

Acrylamide ≥99.9%, white crystalline powder

Catalog Number: (IC04814326)
Supplier: MP Biomedicals
Description: Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules. Common reaction initiators are riboflavin and ammonium persulfate; varying the ratio of acrylamide to crosslinking agent permits the formation of a gel with predictable average pore size and texture. Acrylamide tends to hydrolyze under acidic or basic conditions to form acrylic acid. Polyacrylamide plastics have numerous commercial applications, due in part to the tendency of polyacrylamide to adsorb many times its own mass in water. Acrylic acid and any ionic impurities in the acrylamide can have significant effects on the performance of the acrylamide gel formed, since the voltage across the gel is affected by the ionic charge of the gel and usage buffers.
Store at Room Temperature (15-30 °C), Dessicated.
Image Unavailable-76177-226

2-Mercaptoethanol ≥98%, liquid for molecular biology

Supplier: MP Biomedicals
Description: β-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been commonly used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

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Product Image-PAM7911

GoTaq® Reaction Buffers for PCR, Promega

Supplier: Promega Corporation
Description: Available with green dye for direct-to-gel convenience, or in colorless format for applications where absorbance or fluorescence measurements are necessary prior to PCR cleanup

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Image Unavailable-103010-566

SensoLyte® Green Elastase Assay Kit (Fluorimetric), AnaSpec Inc.

Catalog Number: (103010-566)
Supplier: Anaspec Inc
Description: The SensoLyte® Green Elastase Assay Kit is a FRET-based assay that detects elastase activity. The kit provides an elastin, natural substrate for elastases, labeled with 5-FAM fluorophore and QXL®520 quencher. Elastase-catalyzed hydrolysis yields brightly green fluorescence. Increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase.
Product Image-PAA3511

Reverse Transcriptase System, Promega

Supplier: Promega Corporation
Description: The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in fifteen minutes.

Certificates

Image Unavailable-103010-144

SensoLyte® Green Protease Assay Kit (Fluorimetric), AnaSpec Inc.

Catalog Number: (103010-144)
Supplier: Anaspec Inc
Description: The SensoLyte® Green Protease Assay Kit is widely used for detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugate's fluorescence. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly green fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity (Ex/Em=488/520 nm). The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases.
Product Image-10049-510

Anti-HSP90 Mouse Monoclonal Antibody [clone: 2D11B9]

Catalog Number: (10049-510)
Supplier: Enzo Life Sciences
Description: The 90kDa molecular chaperone family includes 90kDa heat shock protein Hsp90 and 94kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90α are 85% and 90% homologous to those of Hsp90β, respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signalling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophilia Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits, even after Hsp90 function is restored.
Product Image-95016-746

Sephadex™ G-10 Gel Filtration Media, Cytiva

Supplier: Cytiva
Description: Sephadex G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.

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Product Image-10049-530

Anti-HSP91 Rat Monoclonal Antibody

Supplier: Enzo Life Sciences
Description: The 90kDa molecular chaperone family includes 90 kDa heat shock protein Hsp90 and 94 kDa glucose-regulated protein grp94, both major molecular chaperones of the cytosol and the endoplasmic reticulum. Mammalian cells contain isoforms Hsp90α and Hsp90β, encoded by separate genes. The amino acid sequences of human and yeast Hsp90-alpha are 85% and 90% homologous to those of Hsp90β , respectively. All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions containing independent chaperone sites with different substrate specificity. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 functions as part of the cell’s powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. In the absence of stress, however, Hsp90 provides a necessary component of such fundamental cellular processes as hormone signaling and cell cycle control. In this context, researchers identified key regulatory proteins as substrates of Hsp90, including steroid receptors, cell cycle kinases involved in signal transduction, and p53. Hsp90 may act as a capacitor for morphological evolution by buffering widespread variation, potentially affecting morphogenic pathways. When temperature and other stress factors compromise Drosophila Hsp90 buffering, cryptic variant expression occurs, and selection can lead to the continued expression of these traits even after Hsp90 function is restored.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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