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Catalog Number: (97061-558)
Supplier: VWR
Description: A valuable plasma stain used in contrast to hematoxylin. Also a counterstain to safranin.

Supplier: Spectrum Chemicals
Description: Light Green SF Yellowish is a green triarylmethane dye. It is used in histology for staining collagen. It appears as brownish-red to purple powder and is soluble in cold water.

Small Business Enterprise

Supplier: Electron Microscopy Sciences
Description: Light Green SF Yellowish is a high quality ready to use solution. The solution is available as 0.2% in 0.2% acetic acid for use in the GM Method, as a fungal stain and in a 1% aqueous solution.

Minority or Woman-Owned Business Enterprise

Catalog Number: (100504-078)
Supplier: Electron Microscopy Sciences
Description: Grocott's method for fungi. Dahl's method as a bone and calcium stain. McManus' method for glycogen. Fraser-Lendrum method as a connective tissue stain.

Minority or Woman-Owned Business Enterprise


Catalog Number: (101410-984)
Supplier: Electron Microscopy Sciences
Description: 1% Aqueous

Minority or Woman-Owned Business Enterprise


Supplier: Thermo Scientific Chemicals
Description: Light green SF yellowish, pure, high purity biological stain

Supplier: Thermo Scientific Chemicals
Description: Useful as a general plasma stain, as a histological stain, and a cytological counterstain
Catalog Number: (EM1.15941.0100)
Supplier: MilliporeSigma
Description: Cas Number: 5141-20-8, 100G

SDS


Catalog Number: (220195-142)
Supplier: Sigma Aldrich
Description: 25G CAS 5141-20-8

Blocked

Catalog Number: (TCL0054-025G)
Supplier: TCI America
Description: CAS Number: 5141-20-8
MDL Number: MFCD00012121
Molecular Formula: C37H36N2O9S3
Molecular Weight: 792.84
Form: Crystal
Color: Deep Red

SDS


Catalog Number: (TCL0141-001G)
Supplier: TCI America
Description: [as an indicator for assay of Food Yellow No.4 (Tartrazine)]
CAS Number: 5141-20-8
MDL Number: MFCD00012121
Molecular Formula: C37H36N2O9S3
Molecular Weight: 792.84
Form: Crystal
Color: Red

Supplier: Ambeed
Description: Sodium 3-((-ethyl(-4-((4-(ethyl(3-sulfonatobenzyl)amino)phenyl)(4-sulfonatophenyl)methylene)cyclohexa-2,5-dien-1-ylidene)ammonio)methyl)benzenesulfonate, Purity: 97%, CAS Number: 5141-20-8, Red to Dark red to Dark purple powder to crystal, Storage: Inert atmosphere, Room Temperature, Size: 5G

Supplier: VWR
Description: Part Fleming Triple stain, a chromosome stain prepared with light green SF and in Gram stain.
Supplier: MP Biomedicals
Description: Metronidazole ia an antiprotozoal (Trichomonas), a limited spectrum antibiotic that actively deters the growth of protozoa, anaerobic gram-positive, and anaerobic gram-negative bacteria.
Supplier: MilliporeSigma
Description: Used to demonstrate the differentiation of squamous cells in gynecological cytology

SDS

Supplier: Affymetrix, Inc.
Description: + cells. The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability. PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259). Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (cat. 00-5523). Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4 °C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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