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Supplier: Adipogen
Description: Derivative of JC-1 useful for determining mitochondrial membrane potential by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. Accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (570 nm) in healthy cells. Upon cell injury, as membrane potential decreases, JC-10 monomers are generated, resulting in a shift to green emission (520 nm). Shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. Spectral data: lambdaex 510 nm| lambdaem 525.

Catalog Number: (76483-562)
Supplier: AAT BIOQUEST INC
Description: JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase.

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Catalog Number: (89166-016)
Supplier: Enzo Life Sciences
Description: Mitochondria dye


Supplier: Adipogen
Description: JC-10 is superior analog of JC-1, useful for determining mitochondrial membrane potential (MMP) in cells by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-10 is a cationic, lipophilic dye that accumulates in mitochondria of cells with a polarized mitochondrial membrane, selectively generating an orange J-aggregate emission profile (lambdaex = 540 nm/lambdaem = 590 nm) in healthy cells. Upon cell injury or cell death, as membrane potential decreases, which results in the failure to retain JC-10 aggregates in the mitochondria and JC-10 monomers are generated resulting in a shift to green emission (lambdaex = 490 nm/lambdaem = 525 nm). JC-10 shows improved solubility compared to JC-1 in aqueous media and an ability to detect subtler changes in mitochondrial membrane potential loss. JC-10 allows for qualitative visualization (shift from orange to green fluorescence) and quantitative detection (fluorescence intensity ratio) of mitochondrial membrane potential changes. Wavelength Maxima: lambdaex = 510 nm | lambdaem = 525 nm.

Supplier: AAT BIOQUEST INC
Description: Although JC-1 is widely used in many labs, its poor water solubility causes extraordinary inconvenience.

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Catalog Number: (89139-382)
Supplier: Biotium
Description: NAO is a green fluorescent mitochondrial dye whose staining is not dependent on mitochondrial membrane potential.


Catalog Number: (100504-298)
Supplier: Electron Microscopy Sciences
Description: Sodium tungstate can be used as a stain in electron microscopy (Stockert,J.C. (1977), Biol. Cellul., 29:211).

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Supplier: AAT BIOQUEST INC
Description: JC-1 is widely used for determining mitochondrial membrane potential with flow cytometry.

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Supplier: Biosensis
Description: The Biosensis Mouse and Rat proNGF Rapid enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants, cell lysates and tissue homogenates only if used as directed. This ELISA Kit has also successfully been used on mouse urine samples (Ryu JC et al., 2018). Please refer to the kit protocol for specific use instructions for each substrate application.

This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A proNGF positive control (QC sample) is provided to assure consistent assay performance. This ELISA kit detects rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates and rat brain tissue homogenate. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'. This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer.

The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required.

Catalog Number: (89166-014)
Supplier: Enzo Life Sciences
Description: Mitochondria dye


Catalog Number: (80502-824)
Supplier: MilliporeSigma
Description: JC-1, a cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

Catalog Number: (103011-366)
Supplier: Anaspec Inc
Description: Widely used for measuring membrane potential of mitochondria; 585/520 Fluorescence ratio increases upon cell hyper-polarization.


Catalog Number: (76711-210)
Supplier: AFG BIOSCIENCE LLC
Description: Human JC PolyomaVirus IgG(JC-IgG) ELISA Kit


Supplier: Ambeed
Description: JC-1 (iodide) ≥99%

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Catalog Number: (89152-884)
Supplier: Enzo Life Sciences
Description: Fluorescent probe to test Pgp activity


Catalog Number: (89139-380)
Supplier: Biotium
Description: JC-1 (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion.JC-1 monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.


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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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