You Searched For: Hybridization+Buffers


35,236  results were found

SearchResultCount:"35236"

Sort Results

List View Easy View

Rate These Search Results

Catalog Number: (PAG1521)
Supplier: Promega Corporation
Description: Human Female Genomic DNA is purified and stored in 10mM Tris-HCl (pH 8.0), 1mM EDTA >90% of the DNA is longer than 50kb in size as measured by pulsed-field gel electrophoresis. The DNA is suitable for Southern blot hybridizations, genomic analysis (including PCR) and genomic library construction. Human Genomic DNA comes from multiple anonymous donors.

Supplier: Enzo Life Sciences
Description: HPV type 16/18/31/33/51 probe can be optimized for use with polymer detection system or as optimized RTU with Enzo's streptavidin detection methods.

SDS

Supplier: Cytiva
Description: AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).
Catalog Number: (RLBCBT-100)
Supplier: Rockland Immunochemical
Description: BCIP/TNBT Membrane Alkaline Phosphatase Substrate is an artificially manufactured chromogenic substrate for use in sensitive colorimetric assays. BCIP/TNBT can be utilized in ELISA, in situ hybridization, and IHC. Alkaline phosphatase hydrolyzes the BCIP to 5-bromo-4-chloro-3-indole, which is oxidized by oxygen in the atmosphere, yielding a blue dye. In the presence of TNBT, an insoluble dark blue precipitate is formed after a reduction reaction instead.

Small Business Enterprise Minority or Woman-Owned Business Enterprise


Supplier: Adipogen
Description: Fluorescein isothiocyanate (FITC) remains one of the most popular fluorescent labeling reagents. Oligonucleotide conjugates of FITC are frequently employed as hybridization probes. Peptide conjugates of FITC and other fluorescent isothiocyanates are susceptible to Edman degradation, making them useful for high-sensitivity amino acid sequencing, FITC-labeled amino acidd and peptides have been separated by capillary electrophoresis with a detection limit of fewer than 1000 molecules. FITC has also been used to detect proteins in gels and on nitrocellulose membranes and is a selective inhibitor of several membrane ATPases.

Supplier: MP Biomedicals
Description: Storage: Room Temperature
2-Mercaptoethanol is a hybrid of ethylene glycol and 1,2-ethanedithiol. It is used to reduce disulfide bonds and can act as a biological antioxidant by scavenging hydroxyl radicals. It is widely used because the hydroxyl group confers solubility in water and lowers the volatility. Due to its diminished vapor pressure, its odour, while unpleasant, is less objectionable than related thiols.
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also it reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Supplier: VWR International
Description: Hybridization Ovens are designed to provide rapid heat-up, excellent temperature stability, and efficient mixing for all types of hybridization and incubation applications.

UL Listed cUL Listed CSA Certified Product available on GSA Advantage®

Supplier: VWR International
Description: For VWR* Hybridization Oven, Model 5420 (25000-000). Capacity: four 70x220mm (750mL) bottles or four 55x180mm (400mL) bottles.

Supplier: VWR International
Description: Ideal for cell cultures, hybridizations, and suspensions.

Environmentally Preferable Product available on GSA Advantage®

Supplier: VWR International
Description: VWR® Incubating Microplate Shakers feature variable-speed, microprocessor control, providing reliable, uniform mixing action with accurate temperature control you can trust and are ideal for cell cultures, immunoassays, hybridization, genetics research and bacterial suspensions.

New Product

Supplier: VWR International
Description: Ideal for cell culture work, staining and destaining gels, hybridization procedures, hematology, and blotting techniques.

Product available on GSA Advantage®

Supplier: VWR International
Description: VWR® VistaVision™ HistoBond® Adhesive Slides are ideal for <i>in-situ</i> hybridization.
Supplier: Chemglass
Description: Heavy-wall bottles are used in hybridization incubators or ovens with rotators.

Small Business Enterprise

Supplier: VWR International
Description: VWR® shakers feature variable-speed, microprocessor control, providing reliable, uniform mixing action. Ideal for cell cultures, solubility studies, extraction procedures, diagnostic tests, general mixing, hybridization, genetics research, bacterial suspensions, staining/destaining and washing procedures.

New Product

Supplier: VWR International
Description: VWR® Incubating microplate shakers feature variable-speed, microprocessor control, providing reliable, uniform mixing action with accurate temperature control and are ideal for cell cultures, immunoassays, hybridization, genetics research and bacterial suspensions.

New Product

Supplier: VWR
Description: Common reagent for nucleic acid hybridization procedures for detection of DNA/RNA.
Inquire for Price
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
-Additional Documentation May be needed to purchase this item. A VWR representative will contact you if needed.
This product has been blocked by your organization. Please contact your purchasing department for more information.
The original product is no longer available. The replacement shown is available.
This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
65 - 80 of 35,236
no targeter for Bottom