You Searched For: Enzymes

Catalog Number: (76177-422)

Supplier: MP Biomedicals

Description: Lysozyme (muramidase) hydrolyzes preferentially the b-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall structure of certain microorganisms, such as Micrococcus lysodeikticus.

Supplier: Enzo Life Sciences

Description: Produced in <i>E. coli</i>.

Catalog Number: (89152-792)

Supplier: Enzo Life Sciences

Description: Isolated from <i>Salmonella minnesota</i> R595 (Re mutant)

Catalog Number: (89165-712)

Supplier: Enzo Life Sciences

Description: ENZO Proteinase K is specifically designed for use in the pretreatment of formalin-fixed tissue and paraformaldehyde-fixed cells in preparation for in situ hybridization and detection studies.

Catalog Number: (89156-414)

Supplier: Enzo Life Sciences

Description: Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl2, 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.

Catalog Number: (ST300140)

Supplier: Agilent Technologies

Description: Proteinase K is a broad spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA.

SDS

Supplier: MP Biomedicals

Description: Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins and muscle fiber protein.

Supplier: MP Biomedicals

Description: Alcohol dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms. It is a metalloenzyme containing four tightly bound zinc atoms per molecule. Each subunit also contains a second zinc atom (conformational zinc), which stabilizes the enzyme’s tertiary structure. Per subunit, there are two distinct active site sulfhydryl groups which can be distinguished on the basis of differential reactivity with iodoacetate and butyl isocyanate. A histidine residue is considered to have an essential role.

Catalog Number: (76176-668)

Supplier: Corning

Description: Dispase is a bacillus-derived neutral metalloprotease that is recommended for recovering cell cultured on Corning® Matrigel® Basement Membrane Matrix. Dispase will yield a single cell suspension far more gently and effectively than trypsin, collagenase, or other proteolytic enzymes; it will not harm cells harvested for sub cultivation or bioassays. In addition, Dispase may be used for tissue dissociation. Dispase cleaves fibronectin, collagen IV, and to a lesser extent collagen I, but is does not cleave collagen V or laminin.

Certificates

Supplier: MP Biomedicals

Description: Deoxyribonuclease from beef pancreas, DNase I, was first crystallized by Kunitz. It is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding 5'-phosphate terminated polynucleotides with a free hydroxyl group on position 3'. The average chain of limit digest is a tetranucleotide. DNase I acts upon single chain DNA, and upon double-stranded DNA and chromatin.

SDS Certificates

Supplier: MP Biomedicals

Description: One unit will liberate 1.0 µmole of N-acetyl-neuraminic acid (NANA) per minute at pH 5.0 at 37°C, using either one NAN-lactose, bovine submaxillary mucin, or colominic acid as a substrate.

SDS

Supplier: MP Biomedicals

Description: Thrombin is prepared from fresh bovine plasma by an ion exchange method

Certificates

Catalog Number: (IC19068001)

Supplier: MP Biomedicals

Description: Dissolves readily at 5 mg/mL in a 0.025M glychine/NaOH pH 9.6 containing 1 mM magnesium chloride, 0.1 mM zinc chloride and 10% glycerol, to give a clear pale pink solution also soluble in distilled water or dilute buffer.

Catalog Number: (IC15234010)

Supplier: MP Biomedicals

Description: Readily soluble in water.

SDS

Catalog Number: (IC15127080)

Supplier: MP Biomedicals

Description: Unit Definition: Enzyme activity is determined by a change in optical density in a solution of hyaluronic acid. Activity is compared to a hyaluronidase International Standard and is expressed as an International Unit (U).

SDS

Supplier: MP Biomedicals

Description: For Continuous Spectrophotometric Rate Determination method: 0.3 to 0.6 unit/ml of L-Glutamic dehydrogenase in cold Triethanolamine buffer.

SDS