You Searched For: Dithiothreitol+(DTT,+Cleland\'s+reagent)

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SensoLyte® AMC Calpain Activity Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-506)

Supplier: Anaspec Inc

Description: The calpains are a family of intracellular Ca<sup>2+</sup> supdependent cysteine proteases

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SensoLyte® 520 Calpain Activity Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-504)

Supplier: Anaspec Inc

Description: The calpains are a family of intracellular Ca<sup>2+</sup> dependent cysteine proteases

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SensoLyte® 520 IDE Activity Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-678)

Supplier: Anaspec Inc

Description: Insulin degrading enzyme (IDE) is considered a physiological and pathological relevant enzyme

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SensoLyte® 520 Cathepsin D Assay Kit Fluorimetric, AnaSpec, Inc.

Catalog Number: (103010-544)

Supplier: Anaspec Inc

Description: Cathepsin D is the lysosomal aspartic proteinase, active in intracellular protein breakdown

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SensoLyte® 520 TEV Activity Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-670)

Supplier: Anaspec Inc

Description: Tobacco Etch Virus (TEV) proteinase is widely used to remove fusion tags from recombinant proteins

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SensoLyte® Homogeneous AMC Caspase-3/7 Assay Kit, AnaSpec Inc.

Catalog Number: (103010-138)

Supplier: Anaspec Inc

Description: Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7

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SensoLyte® 520 Deubiquitination Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-614)

Supplier: Anaspec Inc

Description: Deubiquitinating enzymes (DUBs) are proteases that reverse ubiquitin modifications and can rescue a protein from proteasomal degradation

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7-Azido-4-methylcoumarin 98

Supplier: Adipogen

Description: Highly sensitive and selective fluorogenic H2S probe. The aromatic azide moiety of AzMC is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with lambdaex = 365 nm and lambdaem = 450 nm. Photoaffinity labeling probe for the substrate binding site of human sulfotransferase 1A1 (SULT1A1). Probe to monitor the enzymatic production of H2S in vitro and to visualize H2S in living cells. Tool for monitoring the activity of pyridoxal-5'-phosphate (PLP)-dependent enzymes (e.g. cystathionine beta-synthase (CBS), cystathionine gamma-lyase (CGL) and tryptophan synthase (TS)). Tool to identify novel cystathionine beta-synthase (CBS) inhibitors and activators. Suitable for high-throughput screening. Caution: Use of this product with DTT, TCEP and/or biological thiols at concentrations of >25 mM should be avoided for maximum efficiency.

Urea ≥98%, white powder for molecular biology

Supplier: MP Biomedicals

Description: Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

SensoLyte® 520 HIV Protease Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-178)

Supplier: Anaspec Inc

Description: The SensoLyte® 520 HIV Assay Kit uses a new FRET peptide substrate that incorporates HiLyte™ Fluor 488 (fluorophore) and QXL® 520 (quencher) for continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of HiLyte™ Fluor 488 is quenched by QXL® 520. Upon cleavage of the FRET peptide by HIV protease, the fluorescence of HiLyte™ Fluor 488 is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this HiLyte™ Fluor 488/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components, thus providing better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.

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SensoLyte® 520 HCV Protease Assay Kit (Fluorimetric), AnaSpec

Catalog Number: (103010-174)

Supplier: Anaspec Inc

Description: The SensoLyte® 520 HCV Assay Kit uses a new FRET peptide substrate that incorporates 5-FAM (fluorophore) and QXL® 520 (quencher) for a continuous measurement of enzyme activities. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL® 520. Upon cleavage of the FRET peptide by HCV NS3/4a protease, the fluorescence of 5-FAM is recovered and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, this 5-FAM/QXL® 520-based FRET peptide shows less interference from autofluorescence of test compounds and cellular components. This assay is ten times more sensitive than an EDANS/DABCYL based assay and can detect 0.1 pmole of HCV NS3/4a protease. The assays are performed in a convenient 96-well or 384-well microplate format.

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