Dithiothreitol (DTT, Cleland's reagent) for biotechnology
Supplier: VWR
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Warning
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0281-25G
0281-5G
97061-338CS
5296.86
USD
97061-338
97061-340
Dithiothreitol (DTT, Cleland's reagent) for biotechnology
Dithiothreitol (DTT, Cleland's reagent)
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
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Formula:
C₄H₁₀O₂S₂ Boiling Pt: ? 125 °C (1013 hPa) Melting Pt: 42 C TO 43 °C Flash Pt: ≥ 110 °C () Storage Temperature: Refrigerator |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 |
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Specification Test Results
| Abs.@283nm (0.02M, Water) | <= 0.05 |
| DNase | none detected |
| Loss on Drying | < 0.5 % |
| Melting Point | 39 °C - 43 °C |
| Oxidized DTT | < 0.5 % |
| Protease | none detected |
| Purity (-SH Content) | > 99.4 % |
| RNase | none detected |
| Solubility (5%, Water) | pass |
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