You Searched For: Dithiothreitol (DTT, Cleland's reagent)

Supplier: VWR

Description: An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).

SDS Certificates

Supplier: VWR

Description: Dithiothreitol (DTT, Cleland's reagent)

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Catalog Number: (JTF781-5)

Supplier: AVANTOR PERFORMANCE MATERIALS US

Description: White crystals. Keep material refrigerated between 2 to 8°C.

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Supplier: AVANTOR PERFORMANCE MATERIALS US

Description: 1,4-Dithiothreitol (DTT, Cleland's Reagent), Ultrapure Bioreagent

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Catalog Number: (IC856126)

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

SDS

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

DTT has been used:

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Supplier: Enzo Life Sciences

Description: Protective agent for SH groups

Supplier: Thermo Scientific Chemicals

Description: Dithiothreitol (DTT, Cleland's reagent) 99% for biochemistry

Supplier: Thermo Scientific Chemicals

Description: Dithiothreitol (DTT, Cleland's reagent) 99+% for molecular biology DNase-, RNase-, protease-free

Supplier: Thermo Scientific Chemicals

Description: Dithiothreitol (DTT, Cleland's reagent) 98%, pure

Supplier: MP Biomedicals

Description: DL-Dithiothreitol is a Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.

SDS

Supplier: RPI

Description: Protective reagent for maintaining -SH groups in reduced state.

Supplier: TCI America

Description: CAS Number: 3483-12-3
MDL Number: MFCD00004877
Molecular Formula: C4H10O2S2
Molecular Weight: 154.24
Purity/Analysis Method: >98.0% (T)
Form: Crystal
Boiling point (°C): 130
Melting point (°C): 43
Flash Point (°C): 113
Storage Temperature: 0-10°C

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Supplier: Thermo Scientific Chemicals

Description: Useful for stabilizing sulfhydryl-containing enzymes

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Supplier: TCI America

Description: CAS Number: 3483-12-3
MDL Number: MFCD00004877
Molecular Formula: C4H10O2S2
Molecular Weight: 154.24
Purity/Analysis Method: >98.0% (T)
Form: Crystal
Boiling point (°C): 130
Melting point (°C): 43
Flash Point (°C): 113
Storage Temperature: 0-10°C

SDS Certificates

Supplier: Thermo Scientific Chemicals

Description: Dithiothreitol (DTT, Cleland's reagent) 1 M in water for biochemistry