CellAdhere™ Laminin-521

Supplier: STEMCELL Technologies

77003 200-0117
MSPP-77003EA 140.31 USD
MSPP-77003 MSPP-200-0117
CellAdhere™ Laminin-521
Cell Culture Supplements and Additives

Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media.


  • Decrease sources of variability in experiments with a recombinant human protein matrix
  • Passage cells without the need for apoptotic inhibitors
  • Use with any TeSR™ family medium to maintain hPSCs
  • Increase single-cell attachment and survival when using with eTeSR™ for single-cell passaging
  • Mimic the stem cell niche with this physiologically-relevant matrix


Support the growth and differentiation of human embryonic stem (ES) and induced pluripotent stem (iPS) cells under feeder-free conditions with this defined and xeno-free cell culture matrix.


For consistent cell populations and reproducible results in downstream applications, use CellAdhere™ Laminin-521 with TeSR™ maintenance media to provide a defined culture substrate for cell maintenance. Laminin 521 is expressed and secreted by human pluripotent stem cells (hPSCs) in the inner cell mass of the embryo and therefore creates a biologically relevant hPSC culture environment in vitro. Use CellAdhere™ Laminin-521 with eTeSR™ (MSPP-100-1215) maintenance medium for single-cell passaging. Compared to other matrices, CellAdhere™ Laminin-521 increases single-cell attachment and survival and does not require the addition of apoptotic inhibitors during long term culture. Pair with Gentle Cell Dissociation Reagent (GCDR;MSPP-07174) or ReLeSR™ (MSPP-05872) for routine passaging of PSC aggregates, or Accutase™ (MSPP-07920) for single-cell passaging workflows.


Note that single-cell passaging of human ES and iPS cells can result in selective pressure and lead to genetic aberrations. If passaging as single cells, we recommend checking the karyotype frequently.


Precaución: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier’s restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

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