Dithiothreitol (DTT, Cleland's reagent) ≥99.0%, white powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
|
Warning
|
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
DTT has been used:
- as one of the reactants in the reduction and alkylation of αs1-Casein, the major allergen of cow′s milk.
- as a component of medium for the demembranation and reactivation of spermatozoa.
- to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.
- as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).It is used in preparation of carboxymethylated rPA (IAA-rPA)(Alexander Tischer(2010), l-Arginine hydrochloride increases the solubility of folded and unfolded recombinant plasminogen activator rPA, Protein science,19(9), 1783-1795 ).
Soluble in water (50 mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
Precaución: Research Use Only (RUO).
|
Formula:
C₄H₁₀O₂S₂ Boiling Pt: ∼125 °C (1013 hPa) Melting Pt: 40…50 °C (lit.) Flash Pt: >110 °C Storage Temperature: Refrigerator |
MDL Number:
MFCD00004877 CAS Number: 27565-41-9 EINECS: 222-468-7 UN: 3335 ADR: 9,III |
|
|
Specification Test Results
| Identity Test | Passes |
| Purity (Titration) | ≥99.0% |
| Melting Point | 40 - 50 °C |
| FTIR | Conforms to Standard |
| OD Readings | For Information Only |
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...