VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific

Supplier: ReVacc Scientific

N31O81-200UL N31O81-1ML
76515-516EA 643.72 USD
76515-516 76515-518
VSV-Pseudovirus_Marburg Ravn Luciferase, ReVacc Scientific
Vectors, Plasmids and Libraries

This pseudotyped virus uses recombinant Vesicular Stomatitis Virus (rVSV) to carry GP protein of Marburgvirus - Ravn (GenBank: DQ447649.1).


Infection of cells with this pseudotyped virus carrying luciferase reporter results in high level luciferase activity. See the titration result showing the undiluted pseudovirus generates signal ~100000-fold higher than uninfected control (cell alone as background). The neutralizing activity of one published mAb called MR191 was tested by using this pseudotyped virus with a HIV control mAb.


As pseudovirus infectivity of rVSV without its original G is restricted to a single round of replication, the pseudotypes can be handled using BSL-2 containment practices. Applications Anti- Marburgvirus neutralizing antibody screening at high throughput, Anti- Marburgvirus drug screening at high throughput and Marburgvirus pseudovirus transduction of target cells for viral entry and functional studies.


Envase: Shipping with dry ice. Require −80 °C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only one cycle. Aliquot after the first thaw.


Precaución: Handle it in biosafety cabinet in BSL-2. Contacted tips and tubes should be decontaminated by 10% disinfecting bleach. For research use only.

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