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You Searched For: Vectors, Plasmids and Libraries

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.

In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.


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Product Image-95040-274

Polyadenylic acid (poly-A)

Supplier: Cytiva
Description: Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.

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AAV Helper-Free System, Agilent Technologies

Supplier: Agilent Technologies
Description: The AAV Helper-Free System eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer. This system works with a broad range of hosts and infects both dividing and non-dividing cells.

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Product Image-99900-962

InterPlay Adenoviral TAP System, Agilent Technologies

Supplier: Agilent Technologies
Description: The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

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Expression vectors, pGEX

Supplier: Cytiva
Description: The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.

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AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The AAV helper-free system eliminates the requirement for wild-type adenovirus co-infection from both AAV vector production and AAV stock titering steps, making this system entirely helper virus-free and safer.

Product Image-89157-406

pJY2 Plasmid, Enzo Life Sciences

Catalog Number: (89157-406)
Supplier: Enzo Life Sciences
Description: pJY2 encodes tRNAUCUArg to suppress lysine misincorporation at AGA codons and T7 lysozyme to depress constitutive expression of the cloned gene. pJY2 can also be used as a source of tRNAUCUArg with expression systems, such as the pGEX series of vectors, that do not rely on T7 polymerase.
Product Image-99900-938

AdEasy Adenoviral Vector Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The AdEasy system saves you a month of work over traditional methods by producing the recombinant adenoviral plasmid by homologous recombination in E. coli. The AdEasy XL system includes BJ5183 cells pre-transformed with the pAdEasy-1 plasmid, this feature dramatically decreases background caused by the non-recombinant shuttle plasmid.

Product Image-95017-592

Vector, E. coli BL21, Cytiva

Catalog Number: (95017-592)
Supplier: Cytiva
Description: The <i>E. coli </i>BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.

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Product Image-95040-832

pBlueScript II Vectors, Agilent Technologies

Supplier: Agilent Technologies
Description: The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.

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Product Image-76193-670

SureVector, Vectors, Agilent Technologies

Supplier: Agilent Technologies
Description: Combine standard DNA components to build custom vector constructs from Agilent’s set of standard parts.

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Vector, pUC 18, MP Biomedicals

Catalog Number: (IC821226)
Supplier: MP Biomedicals
Description: pUC 18 is a small vector of approximately 2.7 Kb containing the Pvu II/EcoR I fragment of pBR322 and the lacZ gene multiple cloning site from M13mp18.

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InterPlay Adenoviral TAP Systems, Agilent Technologies

Supplier: Agilent Technologies
Description: The InterPlay Adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System, for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

Product Image-PAN1111

pNL3.2.NF-kB-RE[NlucP/NF-kB-RE/Hygro] Vector, 20 µg, Promega

Catalog Number: (PAN1111)
Supplier: Promega Corporation
Description: NanoLuc (Nluc) luciferase is a 19.1kDa luminescent reporter enzyme that is about 100-fold brighter than either firefly or Renilla luciferase. Use the pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] Vector to measure changes in the levels of NF-kappaB in cells.

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Product Image-PAE4611

pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector, 20 µg, Promega

Catalog Number: (PAE4611)
Supplier: Promega Corporation
Description: pGL4.44[luc2P/AP1 RE/Hygro], pGL4.49[luc2P/TCF-LEF RE/Hygro], pGL4.29[luc2P/CRE/Hygro], pGL4.30[luc2P/NFAT-RE/Hygro], pGL4.32[luc2P/NF-κB-RE/Hygro], pGL4.33[luc2P/SRE/Hygro] and pGL4.34[luc2P/SRF-RE/Hygro] Vectors can explore various signaling pathways.

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Product Image-PAN1021

pNL1.3[secNluc] Vector, 20 µg, Promega

Catalog Number: (PAN1021)
Supplier: Promega Corporation
Description: The pNL1.3[secNluc], pNL3.3[secNluc/minP], pNL2.3[secNluc/Hygro] and pNL1.3.CMV[secNluc/CMV] Vectors offer a secreted small luciferase reporter in various promoter-driven or promoterless configurations for expression in mammalian cells.

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Product Image-PAE3671

pGL4.48[luc2P/SBE/Hygro] Vector, 20 µg, Promega

Catalog Number: (PAE3671)
Supplier: Promega Corporation
Description: The pGL4.45[luc2P/ISRE/Hygro], pGL4.47[luc2P/SIE/Hygro], pGL4.48[luc2P/SBE/Hygro] and pGL4.52[luc2P/STAT5RE/Hygro] Vectors are optimized for expression in mammalian cells. Use these vectors to explore cytokine signaling pathways.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
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