Lenti-Pseudovirus_SARS-CoV Luciferase, ReVacc Scientific
Supplier: ReVacc Scientific
This pseudotyped virus uses Lentiviral vectors to package full-length S protein of SARS-CoV (2003 strain) (GenBank: DQ412594.1).
Infection of cells with this pseudotyped virus carrying luciferase reporter results in detectable luciferase activity. See the titration result showing the undiluted pseudovirus generates signal ~10000-fold higher than uninfected control (cell alone as background). The neutralizing activity of one control mAb and two published neutralizing mAbs (CR3022 and 80R) was tested by using this pseudotyped virus.
The pseudotypes should be handled using BSL-2 containment practices. Applications include Anti-SARS-CoV-2 neutralizing antibody screening at high throughput, Anti-SARS-CoV-2 drug screening at high throughput and SARS-CoV-2 pseudovirus transduction of target cells for viral entry and functional studies.
Envase: Shipping with dry ice. Require −80 °C storage. Multiple freeze/Thaw cycles will reduce its sensitivity. Recommend only one cycle. Aliquot after the first thaw.
Precaución: Handle it in biosafety cabinet in BSL-2. Contacted tips and tubes should be decontaminated by 10% disinfecting bleach. For research use only.
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