CytoSoft® Tunable Substrate Stiffness Elastic Modulus Plates, Advanced BioMatrix

Supplier: ADVANCED BIOMATRIX, INC. MS
5140-5EA 5141-5EA 5142-5EA 5143-5EA 5144-5EA 5145-5EA 5165-5EA
103700-666PK 209.23 USD
103700-666 103700-668 103700-670 103700-672 103700-674 103700-676 103701-218
CytoSoft® Tunable Substrate Stiffness Elastic Modulus Plates, Advanced BioMatrix
Microplates Cell Culture Plates
Advanced BioMatrix products are recognized for purity, functionality, quality and lot-to-lot consistency, allowing for reproducibility in research.

  • Substrate of specific stiffness
  • Each lot is measured and validated
  • Full physiological range available

This CytoSoft® product has an elastic modulus in a standard flat-bottom 6-well plate. The thickness of the silicone gel is uniform with a ~0.5 mm thick layer of silicone in each well that is fully compatible with mammalian cell cultures. The plates are packaged individually, sterilized using irradiation.

The rigidity of the substrate to which cells adhere can have a profound effect on cell morphology and gene expression. CytoSoft® products provide a tool to culture cells on substrates with various rigidities covering a broad physiological range. On the bottom of each well, there is a thin layer of specially formulated biocompatible silicone, whose elastic modulus (rigidity) is carefully measured and certified. The surfaces of the gels in CytoSoft® products are functionalized to form covalent bonds with amines on proteins. This chemical functionalization is stable and the reaction does not require a catalyst, facilitating the coating of the gel surfaces with matrix proteins and plating cells.

The silicone substrates of CytoSoft® products are optically clear and have a low auto-florescence. The layer of silicone in each well is firmly bonded to the bottom of the well. Unlike hydrogels (such as polyacrylamide gels), silicone gels are not susceptible to hydrolysis, do not dry nor swell, are resilient and resistant to tearing or cracking, and their elastic moduli (rigidities) remain nearly unchanged during extended storage periods.

CytoSoft® products accommodate the harvesting of cells using enzymes such as trypsin and collagenase. There is no biochemical breakdown of the substrate during or after enzyme treatment and there are no residuals of the substrate in the sample retrieved from a CytoSoft® plate.

Precaución: This product is for R and D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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