You Searched For: ADVANCED BIOMATRIX, INC. MS

Catalog Number: (103701-188)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Type III Collagen provides structure and strength to connective tissue, is found many places in the body, especially skin, lung, intestinal walls and the walls of blood vessels. Collagen III is initially produced as procollagen, a protein consisting of three pro-alpha1(III) chains that form the triple-stranded, rope-like molecule. After being synthesized, the procollagen molecule is modified by the cell. Enzymes modify the amino acids lysine and proline in the protein strands by adding chemical groups that are necessary for the strands to form a stable molecule and then later to crosslink to other molecules outside the cell. The Type III procollagen molecules are released from the cell and are processed by enzymes that clip small segments off either end of the molecules to form mature collagen. The mature collagen molecules assemble into fibrils. Cross-linking between molecules produces a very stable fibril, contributing to collagen’s tissue strengthening function.

This Type III Collagen product is isolated from human placenta and is purified using a multi-step process with approximately 85% Type III collagen with the remainder being comprised of Type I collagen. The product is supplied as a sterile solution with 10 mg at approximately 1 mg/ml in 0.01 N HCl, pH 2. A Certificate of Analysis is available with the purchase of each product. Type III collagen product is provided in a user-friendly packaging for use and storage. This Type III collagen product is sterile filtered and is supplied as a ready to use solution.

Type III Collagen is typically used as a thin coating on tissue culture surfaces or as a control. Specific instructions are found in the Directions for Use. This product is generally used in vitro as a substrate scaffold to enhance cell attachment, adherence and proliferation. Type III collagen may be used to culture a variety of cell types.

Catalog Number: (103701-726)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Catalog Number: (103700-640)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

PureCol® collagen is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol®, lyophilized form is not recommended for the formation of a solid gel. PureCol® collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Polyethylene Glycol Diacrylate (PEGDA) is a 3500 MW blank slate hydrogel that gels rapidly at room temperature in the presence of a photoinitiator and light. PEGDA is hydrophilic, elastic and can be customized to include a variety of biological molecules.

Catalog Number: (103701-186)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: PEPTITE-2000® is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. This RGD peptide has been specifically engineered to spontaneously adhere to a variety of surfaces such as polystyrene (either tissue culture treated or non-treated), hollow fibers made of cellulose, glass, polycarbonate membranes and many other materials. This binding is mediated via a hydroscopic C-terminal sequence. The RGD motif is present at the N-terminal end of the peptide, allowing for optimal cell attachment via integrin receptors.

PEPTITE-2000® can be substituted as the primary cell attachment component in a chemically defined animal component-free culture system. PEPTITE-2000® is supplied in sterile 5 mg quantities.

Catalog Number: (103700-642)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.

VitroCol® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is sterile filtered and is supplied as a ready to use solution.

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Extralink-Lite PEGDA is a thiol-reactive crosslinker designed for use in HyStem kits.

Catalog Number: (103792-972)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: A Thiol-reactive crosslinker that allows for easy dissolution of HyStem

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Gelin-S is a thiol-modified gelatin that comes as a lyophilized powder. Gelin-S is a component of the HyStem®-C and HyStem®-HP kits, but can be purchased separately here.

Catalog Number: (103701-190)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Collagenase consists of a blend of enzymes including collagenase, caseinase, clostripain and trypsin designed to effectively hydrolyze collagen and dissociate tissues. Various collagenase products have been evaluated specifically for use with Advanced BioMatrix’ s collagen products. This product is offered to improve the viability and functionality of isolated cells from the digested collagen gels.

Collagenase is provided as a lyophilized, sterile powder in a 50 mg package size. After reconstitution, the product is ready-to-use.

Catalog Number: (103701-204)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Poly-L-Ornithine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of ornithine. Poly-L-Ornithine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.

The molecular weight of Poly-L-Ornithine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.

Poly-L-Ornithine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.

Catalog Number: (103701-172)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: PhotoGel® is 1 gram (2 x 500 mg) of lyophilized methacrylated gelatin. PhotoGel® provides 3D gelatin hydrogels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

Catalog Number: (103700-652)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: VitroCol® collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. VitroCol®, lyophilized form is not recommend for the formation of a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is supplied as a sterile, lyophilized powder.

Catalog Number: (103792-908)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: LAP is a water soluble photoinitiator for use in the polymerization of hydrogels or bioinks. LAP is photocrosslinked with 405nm blue light and is often preferred over Irgacure 2959 due to improved cytocompatibility.

Catalog Number: (103700-648)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality, and lot-to-lot consistency, allowing for reproducibility in research.

Catalog Number: (103700-650)

Supplier: ADVANCED BIOMATRIX, INC. MS

Description: Advanced BioMatrix products are recognized for purity, functionality, quality and lot-to-lot consistency, allowing for reproducibility in research.