Calcein AM ≥95% (by HPLC) fluorescent dye

Supplier: Anaspec Inc

Synonyms: Calcein-AM , Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester , Calcein acetoxymethyl ester , Tetrakis(acetoxymethyl) 2,2',2'',2'''-(((3',6'-diacetoxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylene))bis(azanetriyl))tetraacetate

AS-89201

103011-396EA 252.85 USD

103011-396

Calcein AM ≥95% (by HPLC) fluorescent dye

Calcein AM

Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability

Spectral Properties: Abs/Em = 494/517 nm
Storage: -20°C desiccated and protected from light

In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells. Hela cells stained with AnaSpec’s Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204).

Formula: C₄₆H₄₆N₂O₂₃
MW: 994.86 g/mol

MDL Number: MFCD05861516
CAS Number: 148504-34-1

Specification Test Results

Assay (HPLC)	> 95%
λmax(ε)	284±5 nm ( >7,000 M-1cm-1)
Appearance	White solid

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