FLUO 3/AM ≥90% (by TLC)

Supplier: ADIPOGEN CORP MS

Synonyms: 4-(6-Acetoxymethoxy-2,7-dichloro-3-oxo-9-xanthenyl)-4′-methyl-2,2′(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl) ester, 1-[Amino-5-(2,7-dichloro-6-acetomethoxy-3-oxo-3H-xanthen-9-yl)phenoxy]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid pentaacetoxy-methyl ester

CDX-F0033-M001 CDX-F0033-M010
102989-956EA 342 USD
102989-956 102989-958
FLUO 3/AM ≥90% (by TLC)
FLUO 3/AM

Ca2+-indicator fluo-3 for use with visible-light excitation sources in flow cytometry and confocal laser-scanning microscopy. Since about 1996, fluo-3 has also been extensively used in cell-based high-throughput screening assays for drug discovery. Fluo-3 AM is essentially nonfluorescent until it is hydrolyzed intracellularly and/or in the presence of Ca2+. The green-fluorescent emission (~525 nm) of Ca2+-bound fluo-3 is conventionally detected using optical filter sets designed for fluorescein (FITC). As prepared by Minta, Kao and Tsien, fluo-3 was originally reported to undergo an ~40-fold increase in fluorescence upon binding Ca2+.

Formula: C₅₁H₅₀Cl₂N₂O₂₃
MW: 1129.85 g/mol
Storage Temperature: Freezer
CAS Number: 121714-22-5

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Specification Test Results

Source/Host Synthetic.
Purity 90% (TLC)
Appearance/Color Dark red powder.
Solubility Soluble in DMSO.

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