5-CFDA (5-Carboxyfluorescein diacetate) ≥95% (by HPLC)

Supplier: ADIPOGEN CORP MS

Synonyms: 5-Carboxy-di-O-acetylfluorescein, 5-CFDA, 5-Carboxyfluorescein diacetate

CDX-C0039-M025 CDX-C0039-M100 CDX-C0039-G001
102987-602EA 91.38 USD
102987-602 102987-604 102987-606
5-CFDA (5-Carboxyfluorescein diacetate) ≥95% (by HPLC)
5-CFDA (5-Carboxyfluorescein diacetate)

Carboxyfluorescein diacetate (CFDA), which was originally used to measure intracellular pH but was soon adapted for use as a cell viability indicator. Upon hydrolysis by intracellular nonspecific esterases, CFDA forms carboxyfluorescein.


As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells CFDA is moderately permeant to most cell membranes, with uptake greater at pH 6.2 than at pH 7.41. CFDA has been used as a viability probe with a variety of cells, including bacteria, fungi (e.g. Saccharomyces cerevisiae), spermatozoa, natural killer (NK) cells and tumor cells.

Formula: C₂₅H₁₆O₉
MW: 460.39 g/mol
Storage Temperature: Refrigerator
MDL Number: MFCD00036872
CAS Number: 79955-27-4

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Specification Test Results

Source/Host Synthetic.
Purity 95% (HPLC)
Appearance/Color Yellow solid.
Solubility Soluble in DMSO.

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