BCECF AM ester ≥95% (by HPCE) fluorescent pH indicator
Supplier: ADIPOGEN CORP MS
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Warning
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Synonyms:
2′,7′-Bis(2-carboxyethyl)-5(6′)-carboxyfluorescein acetoxymethyl ester, Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid, BCECF AM
Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.
- Spectral properties: λex 490nm; λem 535nm; λex=505nm;λem=520nm in DMSO.
Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium.
Formula:
C₄₂H₄₀O₂₁ MW: 808.69 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00036969 CAS Number: 117464-70-7 |
Specification Test Results
Source/Host | Synthetic. |
Purity | >95% (HPCE) |
Appearance/Color | White to off-white. |
Solubility | Soluble in DMSO, DMF or acetonitrile. |
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