BCECF AM ester ≥95% (by HPCE) fluorescent pH indicator

Supplier: ADIPOGEN CORP MS
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Synonyms: 2′,7′-Bis(2-carboxyethyl)-5(6′)-carboxyfluorescein acetoxymethyl ester, Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-2',7'-dipropanoic acid, BCECF AM

CDX-C0003-M001 CDX-C0003-M005
102987-512EA 254.77 USD
102987-512 102987-514
BCECF AM ester ≥95% (by HPCE) fluorescent pH indicator
BCECF AM ester

Widely-used fluorescent indicator for estimating intracellular pH. This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.


  • Spectral properties: λex 490nm; λem 535nm; λex=505nm;λem=520nm in DMSO.


Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535nm) when the dye is excited at 490nm vs. the emission intensity when excited at 440nm. This approach is amenable to either spectrofluorometry or flow cytometry. BCECF-AM ester can also be used to investigate intracellular changes in other ions, including potassium.

Formula: C₄₂H₄₀O₂₁
MW: 808.69 g/mol
Storage Temperature: Freezer
MDL Number: MFCD00036969
CAS Number: 117464-70-7

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Specification Test Results

Source/Host Synthetic.
Purity >95% (HPCE)
Appearance/Color White to off-white.
Solubility Soluble in DMSO, DMF or acetonitrile.

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