7-Amino-4-methyl-3-coumarinylacetic acid (AMCA-H) ≥90% (by HPLC)
Supplier: ADIPOGEN CORP MS
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Warning
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Synonyms:
AMCA, 2H-1-Benzopyran-3-acetic acid, 7-Amino-4-methylcoumarin-3-acetic acid, Aminomethyl coumarin acetic acid, 2-(7-Amino-4-methyl-2-oxo-2H-chromen-3-yl)acetic acid
AMCA is one of the brightest amine-reactive blue fluorescent dyes useful for immunofluorescence and fluorescent labeling (Ex/Em: 353/455nm). It is quite photostable, and its fluorescence is pH-independent from pH 4 to 10. The properties include a relatively large Stoke's shift and resistance to photobleaching. Reactive AMCA derivatives are used as contrasting probes for double and triple labeling in immunofluorescence microscopy, arrays and in situ hybridization.
NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on antibodies, proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC.
Formula:
C₁₂H₁₁NO₄ MW: 233.22 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00081051 CAS Number: 106562-32-7 |
Specification Test Results
Source/Host | Synthetic. |
Purity |
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Appearance/Color | Brown solid. |
Solubility | Soluble in DMSO or DMF. |
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