2,3-Diaminonaphthalene

Supplier: ADIPOGEN CORP MS

Synonyms: 2,3-Naphthalenediamine, DAN, 2,3-naphthylenediamine

CDX-D0062-G001 CDX-D0062-G005

102987-732EA 213.23 USD

102987-732 102987-734

2,3-Diaminonaphthalene

2,3-Diaminonaphthalene

The 2,3-diaminonaphthalene (DAN) assay is routinely used in the determination of nitrite/nitrate levels in biological fluids and cellular extracts as one indicator of nitric oxide activity. The assay, as reported by Misko, uses the reaction of DAN with NO2- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity (Ex/Em: ~365/415nm). However, detection at 450nm is recommended to avoid fluorescent blanks and increase sensitivity. The fluorescent background of DAN is low for maximum sensitivity. Detection limits of NO achieved by this method are in the high-pM range. 2,3-Diaminonaphtaline (DAN) is used as a derivatisation-reagent for the determination of selenium at low detection limits. The 4,5-benzopiaselenol-complex can be quantified by photometry or GC.

Formula: C₁₀H₁₀N₂
MW: 158.2 g/mol

MDL Number: MFCD00004116
CAS Number: 771-97-1

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