X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside)
Supplier: ADIPOGEN CORP MS
Synonyms:
BCIG, X-GAL, (2S,3R,4S,5R,6R)-2-((5-Bromo-4-chloro-1H-indol-3-yl)oxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol
Molecular biology reagent. Chromogenic substrate for beta-galactosidase. Used in gene cloning together with IPTG (Prod. No. AG-CC1-0002) to indicate whether a cell expresses the beta-galactosidase enzyme, which is encoded by the lacZ gene, in a technique called blue/white screening. Distinguishes between recombinant and non-recombinant plasmids carrying the beta-galactosidase gene. Used in two-hybrid analysis. X-Gal is used to determine E. coli and coliform content in drinking water samples.
Formula:
C₁₄H₁₅BrClNO₆ MW: 408.63 g/mol Storage Temperature: Freezer |
MDL Number:
MFCD00005666 CAS Number: 7240-90-6 |
Specification Test Results
Purity |
|
Appearance/Color | White to off-white powder. Clear, colourless to light yellow in solution. |
Solubility | Soluble in dimethyl formamide. |
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