You Searched For: Electrophoresis Reagents

Catalog Number: (PI23200)

Supplier: Invitrogen

Description: The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

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Catalog Number: (PI23236)

Supplier: Invitrogen

Description: The Pierce™ Coomassie Plus™ Protein Assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

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Catalog Number: (PI22298)

Supplier: Invitrogen

Description: Thermo Scientific Pierce BMPS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short spacer arm (5.9 Å).

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Catalog Number: (PI22305)

Supplier: Invitrogen

Description: Thermo Scientific Pierce MPBH is a long, maleimide-and-hydrazide crosslinker for conjugating sulfhydryls (cysteines) to carbonyls (aldehyde or ketones, such as those formed by oxidation of glycoprotein carbohydrates).

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Catalog Number: (PI28362)

Supplier: Invitrogen

Description: These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.

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Catalog Number: (PI28363)

Supplier: Invitrogen

Description: These space-saving stock solutions are ideal for quick preparation of standard Tris-glycine (pH 8.5) transfer buffer which is used for Western blotting.

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Catalog Number: (PI22308)

Supplier: Invitrogen

Description: Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).

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Supplier: Invitrogen

Description: Thermo Scientific Pierce DSP (Lomant's Reagent) is a water-insoluble, homo-bifunctional N-hydroxysuccimide ester (NHS ester) crosslinker that is thiol-cleavable, primary amine-reactive, and useful for many applications. DSP contains an amine-reactive NHS ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7–9 to form stable amide bonds and releasing N-hydroxy-succinimide. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS ester crosslinking reagents. DSP is non-sulfonated and insoluble in water, so it must first be dissolved in an organic solvent and then added to the aqueous reaction mixture. Because DSP does not possess a charged group, it is lipophilic and membrane-permeable and so useful for intracellular and intramembrane conjugation. A sulfonated analog of DSP (DTTSP) is water soluble. DSS, the non-cleavable analog of the DSP crosslinker is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.

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Catalog Number: (PI22317)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-SMPB is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

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Catalog Number: (PI22615)

Supplier: Invitrogen

Description: Thermo Scientific Pierce L-Photo-Methionine is an analog of L-methionine that can be incorporated into proteins during synthesis and then light-activated to covalently crosslink interacting proteins in cells.

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Catalog Number: (PI22331)

Supplier: Invitrogen

Description: Thermo Scientific Pierce BMB is a mid-length, maleimide crosslinker for covalent, irreversible conjugation between sulfhydryl groups (e.g., protein or peptide cysteines).

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Supplier: Invitrogen

Description: Blocker BSA in PBS or TBS are ready-to-use, 1X PBS or TBS solutions of 10X (10%) bovine serum albumin protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.

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Supplier: Invitrogen

Description: This luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate for Western blotting provides very stable light output especially for documentation with CCD camera imagers with sensitivity in the mid-femto range.

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Catalog Number: (PI62249)

Supplier: Invitrogen

Description: Hoechst 33342 fluorescent stain is a high-quality solution of Hoechst dye for fixed- and live-cell fluorescent staining of DNA and nuclei in cellular imaging techniques.

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Supplier: Invitrogen

Description: Pierce™ Strong Ion Exchange Spin columns enable rapid protein fractionation and sample preparation based on charge differences. The matrix has a highly porous structure with pores larger than 3000 nm, providing proteins easy access to the membrane's charged ligands. Therefore, adsorptive membranes maintain high efficiencies at high-flow rates and when fractionating large biomolecules with small diffusivities.

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Supplier: Invitrogen

Description: This Thermo Scientific Pierce GST Spin Purification Kit contains Pierce Glutathione Spin Columns and buffer components to provide a fast, easy-to-use format for purifying GST-fusion proteins from cellular lysates.

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