You Searched For: Enzymes

Enzymes accelerate, or catalyse, chemical reactions, and they are known to catalyse more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

Enzymes accelerate, or catalyse, chemical reactions, and they are known to catalyse more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.


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Catalog Number: (76176-668)
Supplier: Corning
Description: Dispase is a bacillus-derived neutral metalloprotease that is recommended for recovering cell cultured on Corning® Matrigel® Basement Membrane Matrix. Dispase will yield a single cell suspension far more gently and effectively than trypsin, collagenase, or other proteolytic enzymes; it will not harm cells harvested for sub cultivation or bioassays. In addition, Dispase may be used for tissue dissociation. Dispase cleaves fibronectin, collagen IV, and to a lesser extent collagen I, but is does not cleave collagen V or laminin.

Catalog Number: (TCF0010-025G)
Supplier: TCI America
Description: CAS Number: 9001-33-6

SDS


Catalog Number: (ST300140)
Supplier: Agilent Technologies
Description: Proteinase K is a broad spectrum protease from Tritirachium album that is ideal for preparing chromosomal DNA.

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Supplier: Enzo Life Sciences
Description: Produced in <i>E. coli</i>.

Catalog Number: (89152-792)
Supplier: Enzo Life Sciences
Description: Isolated from <i>Salmonella minnesota</i> R595 (Re mutant)


Catalog Number: (89165-712)
Supplier: Enzo Life Sciences
Description: ENZO Proteinase K is specifically designed for use in the pretreatment of formalin-fixed tissue and paraformaldehyde-fixed cells in preparation for in situ hybridization and detection studies.


Catalog Number: (89156-414)
Supplier: Enzo Life Sciences
Description: Enzyme regulation and kinetics, comparative studies of substrate or inhibitor specificities, cleavage of target proteins. Assay or digest conditions can vary widely, but concentrations for the MMP enzymes can range between 10 and 300nM, or higher. Reaction temperatures can be between 25 and 37°C, and reaction times can range from 10 min to overnight, again depending on application and substrate. A typical assay buffer is 50mM HEPES, pH 7.0, 10mM CaCl2, 0.05% Brij-35. For more information, contact Enzo Life Sciences Technical Support.


Catalog Number: (TS41676-1000)
Supplier: Thermo Scientific Chemicals
Description: 32239102

Catalog Number: (RLMB-118-0100)
Supplier: Rockland Immunochemical
Description: Collagenase Type 1


Catalog Number: (RLMB-122-0050)
Supplier: Rockland Immunochemical
Description: Collagenase Type 1


Catalog Number: (RLMB-124-0050)
Supplier: Rockland Immunochemical
Description: Collagenase Type 3


Supplier: MP Biomedicals
Description: Alcohol dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms. It is a metalloenzyme containing four tightly bound zinc atoms per molecule. Each subunit also contains a second zinc atom (conformational zinc), which stabilizes the enzyme’s tertiary structure. Per subunit, there are two distinct active site sulfhydryl groups which can be distinguished on the basis of differential reactivity with iodoacetate and butyl isocyanate. A histidine residue is considered to have an essential role.

Supplier: MP Biomedicals
Description: Bromelain is a glycoprotein which is a highly active thiol proteinase (non-specific cysteine protease), which splits glycine esters but not lysine esters; it is also a protein-digesting and milk-clotting enzyme. Substrates susceptible to bromelain include all of the common protein materials such as gelatin, casein, gluten, collagen, elastin, globulins and muscle fiber protein.

Catalog Number: (IC15234010)
Supplier: MP Biomedicals
Description: Readily soluble in water.

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Supplier: MP Biomedicals
Description: One unit will liberate 1.0 µmole of N-acetyl-neuraminic acid (NANA) per minute at pH 5.0 at 37°C, using either one NAN-lactose, bovine submaxillary mucin, or colominic acid as a substrate.

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Supplier: MP Biomedicals
Description: Thrombin is prepared from fresh bovine plasma by an ion exchange method
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