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You Searched For: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.


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Product Image-PAV6597

RET (Y791F) Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant human RET (Y791F) (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

SDS

Product Image-PAV4510

CDK6/CyclinD3 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

SDS

Product Image-PAV4058

DDR2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant human DDR2 (467-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. DDR2 is a member of a novel subclass of RTKs containing a distinct extracellular region encompassing a factor VIII-like domain.

SDS

Product Image-PAV5095

ADP-Glo Kinase Assay + PKC beta I Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV5095)
Supplier: Promega Corporation
Description: Recombinant full-length human PKCbeta I was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKCβI is a member of the PKC family (phospholipid-dependent serine/threonine kinase) and is highly related to PKCβ II.

SDS


Product Image-PAV4104

CDK9/CyclinK Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human CDK9 and CyclinK proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK9/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family.

SDS

Product Image-PAV4020

CHK2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.

SDS

Product Image-PAV6593

NUAK2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.

SDS

Product Image-PAV9421

ADP-Glo Kinase Assay + IRAK4 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV9421)
Supplier: Promega Corporation
Description: Recombinant full-length human IRAK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Interleukin-1 receptor-associated kinase 4 is an important mediator in the signal transduction of Toll-like receptor and IL1R family members.

SDS


Product Image-PAV6929

Kinase Selectivity Profiling System: General Panel + ADP-Glo Assay, 24 × 50 reactions, Promega

Catalog Number: (PAV6929)
Supplier: Promega Corporation
Description: Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

SDS


Product Image-PAV9291

ADP-Glo Kinase Assay + ERK2 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV9291)
Supplier: Promega Corporation
Description: Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).

SDS


Product Image-PAV9541

ADP-Glo Kinase Assay + CDK5/p25 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV9541)
Supplier: Promega Corporation
Description: Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.

SDS


Product Image-PAV6926

Kinase Selectivity Profiling System: Other-2, 8 × 50 reactions, Promega

Catalog Number: (PAV6926)
Supplier: Promega Corporation
Description: Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

SDS


Product Image-PAV8001

ADP-Glo Kinase Assay + MINK1 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV8001)
Supplier: Promega Corporation
Description: Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.

SDS


Product Image-PAV9231

ADP-Glo Kinase Assay + NEK2 Kinase Enzyme System, 1 each, Promega

Catalog Number: (PAV9231)
Supplier: Promega Corporation
Description: Recombinant full-length human NEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans.

SDS


Product Image-PAV4476

MLK2 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.

SDS

Product Image-PAV4478

PAK1/CDC42 Kinase Enzyme System, Promega

Supplier: Promega Corporation
Description: Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).

SDS

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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