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Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.

Competent cells are those that have had their cell walls altered to render it easier to incorporate foreign DNA into their interiors. Cells are made competent by a process that uses calcium chloride and heat shock, and cells that are undergoing very rapid growth are made competent more easily than cells in other stages of growth. Factors such as transformation efficiency, genetic markers, and packaging formats will impact the time and effort required for your project, and so too its success. Choose from a number of cell types and formats to ensure optimal function.


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HMS174(DE3), pET Host Strain Competent Cells

Catalog Number: (80030-334)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80030-330

HMS174, pET Host Strain Competent Cells

Catalog Number: (80030-330)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80601-678

Rosetta-gami B(DE3)pLysS Competent Cells

Catalog Number: (80601-678)
Supplier: MilliporeSigma
Description: 0.4ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.

Certificates


Image Unavailable-80601-670

Rosetta-gami* B Competent Cells

Catalog Number: (80601-670)
Supplier: MilliporeSigma
Description: 0.4ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.

Certificates


Image Unavailable-80030-338

HMS174(DE3)pLysS, pET Host Strain Competent Cells

Catalog Number: (80030-338)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-82601-916

VEGGIE BL21(DE3) SINGLES 1KT

Catalog Number: (82601-916)
Supplier: MilliporeSigma

SDS Certificates


Image Unavailable-80030-332

HMS174, pET Host Strain Competent Cells

Catalog Number: (80030-332)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80030-340

HMS174(DE3)pLysS, pET Host Strain Competent Cells

Catalog Number: (80030-340)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80030-832

HMS174 pET Host Strain Competent Cell Set

Catalog Number: (80030-832)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80030-336

HMS174(DE3), pET Host Strain Competent Cells

Catalog Number: (80030-336)
Supplier: MilliporeSigma
Description: pET Host Strain Glycerol Stocks. All pET System host strains* are available separately as standard glycerol stocks. The most widely used strains are also available as pretested competent cells, ready for transformation (see following page).

Certificates


Image Unavailable-80601-680

Rosetta-gami B(DE3)pLysS Competent Cells

Catalog Number: (80601-680)
Supplier: MilliporeSigma
Description: 1ml. Lysogens of bacteriophage lDE3, Suitable for production of protein from target genes cloned in pET vectors. pLysS designation is given to hosts carrying a pET-compatible plasmid that encodes T7 lysozyme. Suppress basal expression of T7 RNA polymerase.

Certificates


Image Unavailable-80601-674

Rosetta-gami* B Competent Cells

Catalog Number: (80601-674)
Supplier: MilliporeSigma
Description: 0.4ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.

Certificates


Image Unavailable-80601-672

Rosetta-gami* B Competent Cells

Catalog Number: (80601-672)
Supplier: MilliporeSigma
Description: 1ml. Host competent cells that combine the key features of BL21 (& Tuner* derivative), Origami, and Rosetta strains to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm.

Certificates


Image Unavailable-80601-676

Rosetta-gami* B Competent Cells

Catalog Number: (80601-676)
Supplier: MilliporeSigma
Description: 1ml. Host competent cells are isogenic with the lDE3 lysogens used for protein expression with pET, pETBlue*, and pTriEx* vectors, but lack a source of T7 RNA polymerase. Standard host used for initial cloning of pET, pETBlue and pTriEx vector constructs.

Certificates


Product Image-101641-600

E.Coli Ribosome - 1 MG

Catalog Number: (101641-600)
Supplier: New England Biolabs (NEB)

Small Business Enterprise


Product Image-77574-024

COMPETENT CELLS DH5 ONE-MINUTE 400UL PK8

Catalog Number: (77574-024)
Supplier: ReVacc Scientific

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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This product is no longer available. Alternatives may be available by searching with the VWR Catalog Number listed above. If you need further assistance, please call VWR Customer Service at 1-800-932-5000.
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