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You Searched For: Cloning Kits and Ligation

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.

The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.


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Product Image-76081-602

Ampligase* Enzyme and Buffer 750 Units

Description: Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensiti
Catalog Number: 76081-602
Supplier: Lucigen
Product Image-76081-596

Ampligase* 10X Reaction Buffer 5 ml

Description: Ampligase* 10X Reaction Buffer, High specificity and stringency permits sensitive detection of SNPs11, igase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA stru
Catalog Number: 76081-596
Supplier: Lucigen
Product Image-76081-598

Ampligase* Enzyme and Buffer 2,500 Units

Description: Ampligase* Enzyme and Buffer, Ligase catalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are stable at high temperatures, High specificity sensi
Catalog Number: 76081-598
Supplier: Lucigen
Product Image-76081-604

Ampligase* DNA Ligase Kit 1,000 Units

Description: Ampligase* DNA Ligase Kit, catalyzes NAD-dependent ligation, Contains: Ampligase DNA Ligase, 10X Reaction Buffer, and Control DNA. Ampligase Enzyme & Buffer contain: Ampligase DNA Ligase and 10X Reaction Buffer (25 u
Catalog Number: 76081-604
Supplier: Lucigen
Product Image-76081-600

Ampligase* DNA Ligase 10,000 Units

Description: Ampligase* DNA Ligase, for applications where ligation at high temperature is beneficial, High thermostability allows ligation using high-stringency hybridization condition, High specificity and stringency permits sensi
Catalog Number: 76081-600
Supplier: Lucigen
Product Image-76081-592

Ampligase* Thermostable DNA Ligase with Enzyme and Buffer 2,500 Units

Description: Ampligase* Thermostable DNA Ligase with Enzyme and Buffer, atalyzes NAD-dependent ligation of adjacent 3'-hydroxylated and 5'-phosphorylated termini in duplex DNA structures that are s
Catalog Number: 76081-592
Supplier: Lucigen
Product Image-97065-200

NXGEN T4 DNA LIGAS LO CON 100K

Catalog Number: 97065-200
Supplier: Lucigen
Product Image-97065-204

NXGEN T4 DNA LIGASE HIGH CON

Catalog Number: 97065-204
Supplier: Lucigen
Product Image-102715-910

T4 RNA LIgAse 2, Truncated Kq-2,000 Units

Catalog Number: 102715-910
Supplier: New England Biolabs (NEB)

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Product Image-102715-908

T4 RNA LIgAse 2, Truncated Kq-10,000 Units

Catalog Number: 102715-908
Supplier: New England Biolabs (NEB)

Small Business Enterprise


Product Image-PAP9801

Bacterial Strain JM109(DE3), Glycerol Stock

Description: Applications: Synthesis of fusion protein in vivo using pGEMEX recombinant plasmids. Bacterial strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase.
Catalog Number: PAP9801
Supplier: Promega Corporation

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Product Image-101228-190

T4 RNA Ligase

Description: 1,000 units.
Catalog Number: 101228-190
Supplier: New England Biolabs (NEB)

Small Business Enterprise


Product Image-101228-194

E. coli DNA Ligase

Description: 200 units.
Catalog Number: 101228-194
Supplier: New England Biolabs (NEB)

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Product Image-PAM1804

T4 DNA Ligase, 1-3u/ul

Description: Provided with 10X Reaction Buffer: 300mM Tris-HCl (pH 7.8), 100mM MgCl2, 100mM DTT, and 10mM ATP. This buffer should be stored frozen at -20[degree]C in small aliquots to preserve the ATP.
Catalog Number: PAM1804
Supplier: Promega Corporation

Certificates


Product Image-PAM1801

T4 DNA Ligase

Description: Modifying enzyme. For ligation, mutagenesis, cDNA cloning. Concentration: 1-3 units/uL. Size: 100 units. Packaged in a tamper-evident, clear plastic sealed packet that contains the enzyme tube, its buffer(s) and a lot-specific Promega* Product Information insert. 24-month lifetime.
Catalog Number: PAM1801
Supplier: Promega Corporation

Certificates


Product Image-101228-188

T4 RNA Ligase

Description: 5,000 units.
Catalog Number: 101228-188
Supplier: New England Biolabs (NEB)

Small Business Enterprise


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