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RNP transfection reagent – jetCRISPR™

jetCRISPR™ is a RNP delivery reagent, specially designed for co-delivery of guide RNA and cas9 protein for gene editing experiments. jetCRISPR™ leads to high genome editing efficiency while maintaining an excellent cell viability and morphology.

  • Specifically designed for Cas9 protein and guide RNA delivery
  • High genome editing efficiency
  • Excellent cell viability and morphology
  • Fast and reliable gene editing
  • Easy to use: reverse and forward protocols

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jetCRISPR™ has been specifically designed for high transfection efficiency of RNP (guide RNA and Cas9 protein complex), thus leading to a high cas9-mediated genome editing efficiency on a wide variety of targets in different cell types.

High genome editing efficiency using jetCRISPR™ in HeLa cells. RNP transfections were performed in HeLa cells using several RNP concentrations of Cas9 protein and HPRT1 sgRNA or negative control in combination with 0.3 µl of jetCRISPR™ reagent per well of a 96-well plate. At 48 h post-transfection, T7 digestion products were run on a 2% agarose gel and stained with BET displayed by G:Box transilluminator (Syngene ®). Acquisitions were carried out with the Genesnap software (Syngene ®) and INDEL quantifications were performed with the Genetools software (Syngene ®). 1: Uncleaved fragment of 1083 bp, 2: long cleaved fragment of 827 bp, 3: short cleaved fragment of 256 bp.

jetCRISPR™ transfection reagent achieves higher genome editing efficiency than other competitors, which makes it the product of choice for CRISPR applications.

Superior genome editing efficiency obtained with jetCRISPR™ in comparison with Lipofectamine®CRISPRMAX™. RNP transfections were performed in A549 and HEK-293 cells using 30 nM RNP (Cas9 Protein and HPRT1 sgRNA) with 0.3 µl of jetCRISPR™ reagent or 0.3 µl of Lipofectamine ® CRISPRMAX™, per well of 96-well plate. At 48 h post-transfection, genome editing was assessed by calculating the percentage (%) of INDEL using the T7 endonuclease method. The INDEL % was determined by using Genetools software (Syngene ®).

At Polyplus-transfection, we are proud to work with reagents that are extremely gentle to cells, maintaining an excellent cell viability and morphology throughout the whole duration of the experiments. jetCRISPR™ is compatible with both serum and antibiotics, hence RNP transfection can be performed in the optimal conditions for healthy cells.

Excellent cell viability and morphology at 48 h after transfection with jetCRISPR™. RNP transfections were performed on HEK-293 and A549 cells using 30 nM RNP (Cas9 Protein and HPRT1 sgRNA) with 0.3 µl of jetCRISPR™ reagent per well of 96-well plate. At 48 h post-transfection, the cell morphology was visualized by phase contrast ZOE Fluorescent Cell Imager (BIORAD ®).

We now provide a full range of reagents for all your CRISPR experiments:

Product selection

jetCRISPR™ RNP transfection reagent

jetCRISPR™ is an innovative reagent especially designed to deliver RNP in a CRISPR/Cas9 experiment. Use the leading technology for CRISPR/Cas9 RNP delivery!

jetPRIME® DNA transfection reagent

Do you perform transient gene expression? jetPRIME is a powerful and versatile DNA and siRNA transfection reagent for day-to-day experiments that leads to efficient and reliable scientific results.

jetMESSENGER™ mRNA transfection reagent

jetMESSENGER enables transfection in hard to transfect cells using mRNA. Take your gene expression to the next level by switching to mRNA!

in vivo-jetPEI® for in vivo nucleic acid delivery

in vivo-jetPEI is a powerful polymer based reagent used to deliver any nucleic acid to any animal model. in vivo-jetPEI has already been used to target a wide range of organs using various administration routes.