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Supplier: Invitrogen

Description: Thermo Scientific SuperBlock™ Blocking Buffer is optimised solutions in PBS or TBS containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western blot analysis.

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Catalog Number: (PI37587)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Clear Milk Blocking Buffer (10X) is a long shelf-life, high performance replacement for homemade milk blocking buffers in Western blotting applications.

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Catalog Number: (PI84850)

Supplier: Invitrogen

Description: The C18 Spin Tips enable fast and efficient capture, concentration, desalting, and elution of up to 10 µg peptides per 20 µL sample for mass spectrometry (MS) analysis.

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Catalog Number: (PI22312)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Sulfo-MBS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a short aromatic spacer arm (7.3 angstroms).

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Catalog Number: (PI22582)

Supplier: Invitrogen

Description: Thermo Scientific Pierce Ellman's Reagent (DTNB) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

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Catalog Number: (PI22416)

Supplier: Invitrogen

Description: Thermo Scientific Pierce SMPB is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length aromatic spacer arm (11.6 angstroms).

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Supplier: Invitrogen

Description: Thermo Scientific Pierce TCEP-HCl is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as dithiothreitol (DTT), but unlike DTT and other thiol-containing reducing agents, TCEP does not have to be removed before certain sulfhydryl-reactive crosslinking reactions.

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Catalog Number: (PI-15123)

Supplier: Invitrogen

Description: Thermo Scientific NeutrAvidin Protein Coated Clear 96-Well Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays.

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Supplier: Invitrogen

Description: For fast, effective protein and peptide hydrolysis.

Catalog Number: (PI88844)

Supplier: Invitrogen

Description: Magnetic c-Myc-Tag IP/Co-IP kit contains specific immunoaffinity magnetic beads and reagents to perform immunoprecipitation assays of c-Myc fusion proteins or co-IP experiments using c-Myc-tagged bait proteins.

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Supplier: Invitrogen

Description: The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.

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Catalog Number: (PI23246)

Supplier: Invitrogen

Description: The Thermo Scientific™ Pierce™ Detergent Compatible Bradford Assay Kit is a quick and ready-to-use modification of the well-known Bradford coomassie dye-binding, colorimetric method for total protein quantitation

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Catalog Number: (PI84868)

Supplier: Invitrogen

Description: Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.Easy-to-use—resin provided in single-use spin column formatImproved protein identifications—protein identifications increased by ≥50% when compared to unfractionated samplesReproducible—elution profiles and fractional resolution vary by less than 20%Optimized—robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlapCompatible—reagents validated with a variety of complex samples, including TMT™-labeled peptidesIn order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a highpH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10–100 µg of peptide sample using a microcentrifuge.Native, phosphorylated, Tandem Mass TagTM (TMTTM)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.Applications:Reducing sample complexity to identify targets of interestConducting systems biology studiesReducing sample complexity to improve quantitation studies

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Supplier: Invitrogen

Description: The Pierce™ Fast Western Blot Kit, SuperSignal™ West Femto Substrate contains optimised reagents that shorten the time to perform a typical Western blot from 4 hours to ~60 minutes

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Catalog Number: (PI34012)

Supplier: Invitrogen

Description: The 1-Step™ chloronaphthol solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

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Catalog Number: (PI34021)

Supplier: Invitrogen

Description: The Pierce™ TMB Substrate Kit contains soluble TMB (3,3´,5,5´-tetramethylbenzidine) that is ideal for sensitive ELISA-based applications.

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