You Searched For: Western Blot Detection Systems

Catalog Number: (71005-960)

Supplier: VWR

Description: Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting. The enhancer is a ready-to-use solution that replaces TBS-T with non-fat dry milk, or other diluent, for antibody incubation steps. It is formulated for use with HRP- and biotin-conjugated antibodies and works with both PVDF and nitrocellulose blots.

SDS Certificates

Supplier: Cytiva

Description: Amersham Hybond LFP 0.2 PVDF is a low-fluorescent, hydrophobic membrane, with 0.2 µm pore size, for protein transfer. Amersham Hybond LFP is optimized for fluorescent detection in Western blotting applications and for use with the Amersham ECL Plex detection system.

Certificates

Catalog Number: (76545-500)

Supplier: BIOASSAY SYSTEMS

Description: Direct assays of peroxidases, ELISA, immunochromogenic chemistry, western blot.

Supplier: G-Biosciences

Description: The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

SDS

Catalog Number: (95059-140)

Supplier: KPL

Description: Wash solution for use with alkaline phosphatase based detection systems on membranes.

Supplier: GVS FILTER TECHNOLOGY

Description: Supported Nitrocellulose (MCE) membranes.

Catalog Number: (101684-070)

Supplier: Revvity Health Sciences

Description: The Western Lightning™ Chemiluminescence Reagent Plus is a non-radioactive light-emitting system designed to detect proteins immobilized on a membrane. The method provides a sensitivity of 1 - 10 pg of protein and yields fast, permanent, hard copy results on Kodak X-Omat Blue Autoradiography Film or on the Geliance Imaging Systems. Membranes can be stripped and reprobed if stored wet between uses. Western Lightning PLUS is an enhanced luminol-based substrate for HRP-catalyzed detection. It is an economical choice that provides twice the sensitivity of standard reagents, and up to eight times the sensitivity of some brands. Furthermore, it offers the best signal-to-noise ratio, and generates good results on either PVDF or nitrocellulose membranes. PerkinElmer guarantees the performance of Western Blot Chemiluminescence Reagent Plus for 12 months from ship date, and unopened reagent can be stored for at least eight weeks at room temperature. This extended shelf life means you can buy and use Western Blot Chemiluminescence Reagent Plus with confidence.

Supplier: G-Biosciences

Description: G-Biosciences' SWIFT™ Western Diluent is a unique solution that reduces the blocking and antibody incubations on Western blot membranes from >4 hours to <60 minutes

SDS

Supplier: GVS FILTER TECHNOLOGY

Description: Neutral nylon transfer membrane.

Catalog Number: (10793-298)

Supplier: Genetex

Description: Trident Plus Western HRP substrate is a luminol-based, chemiluminescent substrate for the detection of HRP-conjugated antibody on immunoblots at picogram level. It produces an excellent signal that can be easily detected by chemiluminescent imaging methods including X-ray film and chemiluminescence imaging system.

Catalog Number: (RLK915)

Supplier: Rockland Immunochemical

Description: Epitope tag antibody sampler kit detects overexpressed fusion proteins containing the corresponding epitope tags. DYKDDDDK Tag Antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG® antibodies). Epitope tags are useful for the labeling and detection of proteins using western blotting, immunoprecipitation, and immunostaining techniques.

Catalog Number: (101688-072)

Supplier: Revvity Health Sciences

Description: Western Lightning Ultra is a very sensitive chemiluminescence system for detection of horseradish peroxidase (HRP) on membranes, with maximum sensitivity in the low femtogram range. It incorporates a novel enhancer that intensifies a signal generated by peroxidase catalyzed oxidation of luminol. The substrate produces a signal at 425 nm for at least 8 hours, making it ideal for use with a wide variety of CCD imagers as well as Kodak BioMax® Light or X-OMAT Blue film. Membranes may be stripped and re-probed for detection of multiple targets.

Catalog Number: (103489-194)

Supplier: Affymetrix, Inc.

Description: By sandwich ELISA, MA5-14732 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MA5-14732, a biotinylated detection antibody followed by Streptavidin-HRP is recommended. By Western blot, MA5-14732 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14732 is also recommended for detecting endogenous Apo A-1 by Western blot. By Western blot, MA5-14732 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MA5-14732 is also recommended for detecting endogenous Apo A-1 by Western blot. Product MA514732 is a smaller package size of MIA1405 (formerly sold as a Seradyn product). This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis.

Supplier: Cytiva

Description: Enhanced chemiluminescence (ECL) detection reagents for reliable detection of medium and high expression proteins.

SDS Certificates

Supplier: Thermo Scientific Chemicals

Description: For chemiluminecent detection of HRP-based Western blotting systems.

SDS Certificates

Catalog Number: (103471-152)

Supplier: Affymetrix, Inc.

Description: MA3-1000 detects lamin A/C from human, bovine, porcine and canine samples. MA3-1000 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects ~ 70 and 65 kDa proteins corresponding to lamin A and C, respectively, from HeLa cell extract. This antibody can also be used for immunofluorescence and immunohistochemistry for subcellular localization experiments. MA3-1000 has been widely used for detection of lamin A/C control in siRNA experiments. The MA3-1000 antigen is porcine lamin preparation. Lamins are a class of intermediate filament proteins that form a matrix on the inner surface of the nuclear envelope. These proteins are found in many different cell types in three different forms (A, B, and C). Lamins A and C are alternatively spliced versions of the LMNA gene. The LMNA gene has been linked to many disorders of the muscular system, nervous system, and the fat distributions systems including: Emery-Dreifuss muscular dystrophy, Dunnigan-type familial partial lipodystrophy (FPLD), limb-girdle muscular dystrophy (LGMD1B), dilated cardiomyopathy (CMD1A), axonal neuropathy (Charcot-Marie-Tooth disease; CMT2B1), and mandibuloacral dysplasia (MAD).