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Ubiquitin Conjugating Kit (HeLa Lysate-Based), Enzo Life Sciences

Catalog Number: (89165-450)
Supplier: Enzo Life Sciences
Description: 1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection
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RIPA Lysis Buffer, Rockland

Supplier: Rockland Immunochemical
Description: RIPA (Radio-Immunoprecipitation Assay) Lysis Buffer enables rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells

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Product Image-PI89875

Pierce™ ProteoSeek™ Albumin/IgG Removal Kits, Thermo Scientific

Catalog Number: (PI89875)
Supplier: Invitrogen
Description: The Pierce™ Albumin/IgG Removal Kit is optimised to decrease the abundant albumin and antibody components of human serum samples in preparation for 2D electrophoresis and other protein profiling methods. By eliminating most of these two proteins, which account for nearly 70% of total serum proteins, other less abundant proteins of interest can be more easily detected and studied.

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Product Image-PI89874

Pierce™ Mitochondria Isolation Kit for Cultured Cells, Thermo Scientific

Catalog Number: (PI89874)
Supplier: Invitrogen
Description: The Mitochondria Isolation Kit for Cultured Cells provides a versatile, microcentrifuge-tube method for fractionating intact mitochondria from cultured mammalian cell samples.

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Cell Lysates, Rockland Immunochemicals

Supplier: Rockland Immunochemical
Description: Ideal as positive controls for Western blotting, ready-to-use cell lysates are derived from cell lines using highly refined extraction protocols to ensure quality, protein integrity, and lot-to-lot reproducibility

Small Business Enterprise Minority or Woman-Owned Business Enterprise

Product Image-PI90056

Pierce™ Endoproteinase Chymotrypsin Protease (TLCK treated), MS Grade, Thermo Scientific

Catalog Number: (PI90056)
Supplier: Invitrogen
Description: Chymotrypsin Protease, MS Grade, is a purified native endoproteinase validated for maximum activity and selectivity in proteomic and used in in-solution digestions and in-gel digestion of proteins from from 1-D or 2-D gels. Store at -20˚C in a nonfrost-free freezer.

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Product Image-PAL4380

Wheat Germ Extract, Promega

Catalog Number: (PAL4380)
Supplier: Promega Corporation
Description: Wheat Germ Extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors) and is used for in vitro translation..

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ImmunoHistoZyme™, Ready-to-Use, Electron Microscopy Sciences

Supplier: Electron Microscopy Sciences
Description: This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

Product Image-76538-666

NEBExpress® E. coli Lysis Reagent, New England Biolabs

Supplier: New England Biolabs (NEB)
Description: NEBExpress® <i>E. coli</i> Lysis Reagent is a chemical lysis solution composed of a proprietary mix of non-ionic and zwitterionic detergents and Tris-based buffer. It allows disruption of <i>E. coli</i> cells without denaturing soluble proteins.

Product Image-PAL4151

Rabbit Reticulocyte Lysate, Untreated, 1ml, Promega

Catalog Number: (PAL4151)
Supplier: Promega Corporation
Description: Untreated Rabbit Reticulocyte Lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease.

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Product Image-76081-780

Ready-Lyse™ Lysozyme Solution, Degradation of Bacterial Cell Walls, Biosearch Technologies

Supplier: Lucigen
Description: Lyse Gram positive or Gram negative bacteria for protein or nucleic acid purifications from bacteria

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IBI DNA/RNA/Protein Extraction Kit, IBI Scientific

Supplier: IBI Scientific
Description: The DNA/RNA/Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids

Product Image-PAV5072

Trypsin/Lys-C Mix, MS Grade, Promega

Supplier: Promega Corporation
Description: Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

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Product Image-PI87787

Pierce™ IP Lysis Buffer, Thermo Scientific

Supplier: Invitrogen
Description: Pierce™ IP Lysis Buffer is optimized for cell lysate yield, purity and compatibility with immunoprecipitation (IP and Co-IP) as the downstream application for the cell lysate.

SDS Certificates

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Pierce Imject™ Maleimide-Activated Ovalbumin, Thermo Scientific

Supplier: Invitrogen
Description: Imject™ Maleimide-activated ovalbumin is sulfhydryl-reactive ovalbumin (OVA) for hapten-carrier conjugation with cysteine-containing peptides and other thiolated antigens.

SDS

Product Image-PI28340

Pierce™ Surfact-Amps™ Detergent Sampler, Thermo Scientific

Catalog Number: (PI28340)
Supplier: Invitrogen
Description: Thermo Scientific Surfact-Amps Detergent Sampler is a convenient collection of various Thermo Scientific Surfact-Amps Detergent Solutions and selected other useful detergents for protein research methods.

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Stock for this item is limited, but may be available in a warehouse close to you. Please make sure that you are logged in to the site so that available stock can be displayed. If the call is still displayed and you need assistance, please call us at 1-800-932-5000.
This product is marked as restricted and can only be purchased by approved Shipping Accounts. If you need further assistance, email VWR Regulatory Department at Regulatory_Affairs@vwr.com
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